医学部附属病院

西村 基

ニシムラ モトイ  (Nishimura Motoi)

基本情報

所属
千葉大学 医学部附属病院検査部 講師
(兼任)遺伝子診療部 講師
学位
医学博士(2004年3月 千葉大学)

研究者番号
80400969
J-GLOBAL ID
201901001893625153
researchmap会員ID
B000380375

外部リンク

学歴

 1

論文

 123
  • Yuri Matsuki, Kiyoshi Ichihara, Yoshihisa Itoh, Kazuo Mori, Hiroshi Ihara, Masato Maekawa, Motoi Nishimura, Sachiko Kiuchi, Fumio Nomura, Naotaka Hashizume, Nobue Itoh, Satoshi Matsumura
    Clinical nutrition ESPEN 61 119-130 2024年6月  
    BACKGROUND & AIMS: Serum retinol (ROH) is commonly used for population level assessment of vitamin A status. High-performance liquid chromatography (HPLC) is considered most accurate method for measuring ROH. However, with the technical difficulty of using HPLC for routine assays, serum retinol-binding protein (RBP) measured by immunological assays is expected to be a surrogate marker for ROH, with reports of a close correlation between serum RBP and ROH. Nevertheless, RBP is not commonly tested to assess vitamin A status with concerns over RBP alterations under various physiopathological conditions. Thus, we reappraised the extent to which RBP could be used as a surrogate marker in representative disorders that alter serum RBP levels. As a related marker, diagnostic utility of transthyretin (TTR) was also evaluated. METHODS: To evaluate the reliability of ROH and RBP assays, specimen stability was assessed in terms of (1) storage at 25, 4, -20, and -80 °C for 1-28 days, (2) five-cycle freeze-thawing, and (3) fluorescent light exposure for 1-14 days. Sources of variation (sex, age, body mass index [BMI], and drinking habits) and reference intervals for ROH, RBP, and TTR were determined in 617 well-defined healthy individuals. To investigate the influence of disorders that affect serum RBP, patients with five diagnostic groups were enrolled: 26 with chronic kidney disease (CKD); 13 with various malignancies in advanced stages (AdM), 12 with acute bacterial infections (ABI), 6 with liver cirrhosis (LC), and 26 with simple obesity (BMI ≥ 27 kg/m2). RESULTS: The stability of RBP and ROH in serum was confirmed under all conditions. In healthy individuals, serum ROH, RBP, and TTR were appreciably high in males with a slight increase in proportion to age and BMI. The major-axis regression line between RBP (x) and ROH (y) in healthy individuals was y = x, with a correlation coefficient of 0.986. In the LC, AdM, and ABI groups, similar strong correlations were observed; however, the regression lines were shifted slightly rightward from the healthy group line, indicating a positive bias in estimating ROH. Interestingly, the same analyses between TTR and ROH revealed similar strong linear relationships in all groups; however, the regression line of each group showed a leftward (opposite) shift from the healthy group line. Based on these observations, we developed a novel regression model composed of RBP and TTR, which gave much improved accuracy in estimating ROH, even under these pathological conditions. CONCLUSIONS: The perfect RBP-ROH correlation in healthy individuals indicates the utility of RPB as a surrogate marker for ROH. Nevertheless, under RBP-altered conditions, a slight overestimation of ROH is inevitable. However, when the TTR was tested together, the bias can be corrected almost perfectly using the novel ROH estimation formula comprising RBP and TTR.
  • 大野 泉, 西村 基, 大内 麻愉, 菅 元泰, 永嶌 裕樹, 高橋 幸治, 大山 広, 平野 翔, 中島 裟文, 鬼澤 歩, 渡辺 奈未, 宇津野 恵美, 高野 重紹, 市川 智彦, 松下 一之, 滝口 裕一, 加藤 直也
    日本消化器病学会雑誌 120(臨増大会) A842-A842 2023年10月  
  • 大野 泉, 西村 基, 大内 麻愉, 菅 元泰, 永嶌 裕樹, 高橋 幸治, 大山 広, 平野 翔, 中島 裟文, 鬼澤 歩, 渡辺 奈未, 宇津野 恵美, 高野 重紹, 市川 智彦, 松下 一之, 滝口 裕一, 加藤 直也
    日本消化器病学会雑誌 120(臨増大会) A842-A842 2023年10月  
  • 坂井 元春, 石渡 一樹, 五十嵐 活志, 類家 裕太郎, 内藤 久美子, 藤本 真徳, 鈴木 佐和子, 小出 尚史, 横手 幸太郎, 大橋 優美, 岡野 公亮, 西村 基
    千葉医学雑誌 99(4) 106-106 2023年8月  
  • 坂井 元春, 石渡 一樹, 五十嵐 活志, 類家 裕太郎, 内藤 久美子, 藤本 真徳, 鈴木 佐和子, 小出 尚史, 横手 幸太郎, 大橋 優美, 岡野 公亮, 西村 基
    千葉医学雑誌 99(4) 106-106 2023年8月  

MISC

 175

共同研究・競争的資金等の研究課題

 17