研究者業績
基本情報
- 所属
- 千葉大学大学院 医学研究院 環境生命医学 教授 (医学博士)
- 学位
- 博士・乙(京都大学)
- J-GLOBAL ID
- 200901071849698152
- researchmap会員ID
- 1000026429
- 外部リンク
1984年旭川医科大学卒業、同年京都大学医学部助手。カナダマニトバ大学医学部客員講師、米国国立衛生研究所客員研究員、京都大学助教授を経て2000年に千葉大学医学部教授に就任。2001年より千葉大学大学院医学研究院環境生命医学教授。2008年より千葉大学予防医学センター長兼任。専門は、環境生命医学、発生学、解剖学。著書に「鷗外と脚気」（NTT出版）等。
研究キーワード
4研究分野
2経歴
5-
2001年4月 - 現在
-
2008年4月 - 2024年3月
-
2000年4月 - 2001年3月
-
1992年 - 2000年
-
1984年 - 1992年
受賞
3-
2005年
-
2001年
-
1995年
論文
335-
Anatomical science international 87(4) 238-244 2012年12月 査読有り
-
TOXICOLOGY LETTERS 211 S94 2012年6月17日 査読有り
-
Journal of Applied Toxicology 32(2) 98-107 2012年2月 査読有りAzole derivatives have teratogenic effects in rodents. In the present study, malformations and their sensitive windows induced by high-dose ketoconazole (KCZ), an azole derivative, without maternal toxicity were investigated. In addition, the malformation spectrum determined was compared with that induced by vitamin A palmitate (VAP). Pregnant rats were administered a single dose of KCZ by oral gavage on specific individual days from gestational days 8 to 15 (GDs 8-15). Maternal animals were subjected to necropsy on GD 20, and the obtained fetuses were examined for external, visceral and skeletal malformations. The malformation spectrum of VAP was identified from available published data (Noda, Sato, and Udaka, 1982) and a complementary study (single administration of VAP at 1 200 000IU kg -1). Embryonic lethality was observed in dams given KCZ on GDs 9-12 with peak incidence on GDs 10 and 11 with complete resorption. KCZ induced major malformations included cleft palate, digital anomalies, misshapen limbs and unique discontinuous ribs, and the sensitive window for each was identified. Compared with the malformations induced by VAP, unique malformations (e.g. discontinuous ribs by KCZ, neural tube defects by VAP), similar malformations with similar sensitive windows (e.g. digital and limb malformations) and similar malformations with different sensitive windows (e.g. embryonic lethality and cleft palate) were distinguished, suggesting that the mechanisms of several of the types of KCZ-induced malformation are related to excessive vitamin A. © 2011 John Wiley & Sons, Ltd.
-
The Journal of toxicological sciences 37(5) 987-999 2012年 査読有り
-
Allergology International 60(3) 373-379 2011年 査読有りBackground: Environmental exposure to formaldehyde is commonly associated with clinical symptoms such as mucosal irritation and olfactory disorders. However, the impact of such exposure on the development of mucosal inflammation and its outcome has not been carefully evaluated. Methods: The observational non-comparative study was planned. The study population consisted of group of 41 medical students who had signed up for a cadaver dissection course as part of their gross anatomy teaching at the school of medicine Chiba University in Japan. During such dissection course, the students are exposed to variable levels of environmental formaldehyde routinely employed for the preservation the cadavers. The subjects were evaluated by a detailed medical examination. We measured their serum IgE levels. In addition, an olfaction test and nasal mucosal sensitivity to histamine was serially determined, immediately before and after the course and 6 months after the completion of the course. Results: Olfactory abnormalities were observed in 13/41 (32%) subjects and increased nasal mucosal hypersensitivity to histamine was observed in 17/41 (41%) during and immediately after completion of the course. These subjects had evidence of preexisting allergic rhinitis. 6/41 (15%) other students with no prior evidence of allergic rhinitis also exhibited formaldehyde associated clinical symptoms during the dissecting course. However, the symptoms disappeared upon completion of the course in all subjects studied. Conclusions: Temporary abnormalities in the olfaction test and increased nasal mucosal hypersensitivity to histamine were observed in a few students with preexisting allergic rhinitis after environmental exposure of high concentrations of formaldehyde. These effects appeared to be transient. © 2011 Japanese Society of Allergology.
-
CHEMOSPHERE 82(1) 107-113 2011年1月 査読有りWe measured the concentration of each polychlorinated biphenyl (PCB) congener in whole blood, plasma and blood cells, and investigated the distribution of PCBs in human blood using high-resolution gas chromatography/high-resolution mass spectrometry (HRGC/HRMS). The PCB concentrations in plasma and whole blood in terms of lipid concentrations were almost equal, with a correlation coefficient of r = 0.972. In the blood, the ratio of PCBs in blood cells to those in plasma was generally about 1:9 and the congener distribution patterns in blood cells and plasma were similar. We performed verification of a simple mass screening method by obtaining information on the main PCB congeners for investigations on human accumulation and exposure. The total concentration of the seven PCB congeners (UNEP-7) proposed to the United Nations Environment Programme (UNEP) by Muir and Morita was about 50% of the total concentration of all PCB congeners, and UNEP-30 was about 80%. The seven main congeners in the blood (MCB-7) showed a value that was about 60%, and MCB-30 showed a value that was about 90%. Determinations with the main congeners in the blood showed a correlation of r = 0.990 or more between the main eight congeners (MCB-7 plus #74) and the total PCB concentration for all congeners. The results suggest that, although total PCB concentration can be effectively estimated from the main seven congeners, the main eight congeners would be preferable, and that the use of these congeners in the simple mass screening method would be effective for populations in areas uncontaminated by PCBs. (C) 2010 Elsevier Ltd. All rights reserved.
-
EPIDEMIOLOGY 22(1) S81 2011年1月 査読有り
-
EPIDEMIOLOGY 22(1) S46-S47 2011年1月 査読有り
-
EPIDEMIOLOGY 22(1) S41 2011年1月 査読有り
-
臨床環境医学 19(2) 95-104 2010年12月 査読有り自分のPCBs(Polychlorinated biphenyls:ポリ塩化ビフェニール)汚染状況に興味を持った参加者に採血を行い、汚染濃度データを郵送によって告知した。その後、自分の汚染濃度を知った後の心理状況と行動の変化に関するアンケート調査を実施したので結果を報告する。送付対象者144名(男性66名、女性78名)中、97名から回答があった(回収率67.4%)。内、有効回答は71(男性30名、女性41名)、有効回答率49.3%だった。参加動機として多かったものは「自分の汚染濃度を知りたかったから」が男性では70.0%、女性では80.5%、「環境問題に興味があるから」が男性で63.3%、女性で75.6%であった。調査結果を知った時の気持ちで、「最もよくあてはまる」と回答のあった項目は「生活習慣と濃度との関係を知りたい」と答えた人が男性で60.0%、女性で68.3%、「結果を知ってよかった」が男性で53.3%、女性で68.3%だった。調査後の行動については、本研究参加について、家族や友人など他者と「次世代への環境について話した」が男性では40.0%、女性では65.9%だった。本研究参加者は、延べ2000名以上の人にこの研究を話題として示しており、大きな社会的影響力を持つことがわかった。今回の対象者は、検査費を一部負担した上で調査に参加するという、元々環境問題に強い関心を持つ人々が多かったため、この結果をただちに一般化することはできない。化学物質の健康影響問題に関して、研究を通じての社会参加の意識や学習意欲について考察した場合、本研究は、若年層と比べて高齢の世代において、より積極性が高いことを指摘した。しかしながら環境汚染による次世代影響を考えると、より若い世代の男女が関心を持ち行動につなげることがより重要と推測され、今後の働きかけが重要であると考えられた。加えて、自分の汚染度を知ることによって、化学物質による健康影響問題についての関心を引き起こし、結果として生活習慣の見なおしや、周囲の人々に対する影響の波及の可能性が示唆された。(著者抄録)
-
Journal of andrology 31(5) 507-518 2010年9月 査読有り
-
Biology of Reproduction 82(3) 504-515 2010年3月 査読有りMammalian sperm require ATP for motility, and most of it is generated by glycolysis. The glycolytic enzymes segregate to the principal piece region of the flagellum, where some are bound tightly to a novel cytoskeletal structure defining this region, the fibrous sheath (FS), and others are easily extracted with detergents. One of the latter is the spermatogenic cell-specific variant isozyme of hexokinase type 1 (HK1S), characterized by an N-terminal 24-amino acid spermatogenic cell-specific region (SSR). Yeast two-hybrid screens carried out using the SSR as bait determined that HK1S is tethered to muscle-type phosphofructokinase (PFKM) in the principal piece region. This led to the identification of four testis-specific Pfkm splice variants, one that overlapped a variant reported previously (Pfkm-v1) and three that were novel (Pfkm-v2, Pfkm-v3, and Pfkm-v4). They differ from Pfkm transcripts found in somatic cells by encoding a novel 67-amino acid N-terminal extension, the testis-specific region (TSR), producing a spermatogenic cell-specific PFKM variant isozyme (PFKMS). An antiserum generated to the TSR demonstrated that PFKMS is present in the principal piece and is insoluble in 1% Triton X-100 detergent. In subsequent yeast two-hybrid screens, the TSR was found to interact with glutathione S-transferase mu class 5 (GSTM5), identified previously as a spermatogenic cell-specific component of the FS. These results demonstrated that HK1S is tethered in the principal piece region by PFKMS, which in turn is bound tightly to GSTM5 in the FS. © 2010 by the Society for the Study of Reproduction, Inc.
-
Gerodontology 26(4) 250-258 2009年12月 査読有りObjectives: The purpose of this study was to provide an oral function promotion programme for the independent elderly and evaluate the changes in oral health status and oral function. Background: Few studies have scientifically analysed and evaluated the effectiveness of oral function promotion programmes provided for the independent elderly. Materials and methods: The subjects were independent elderly females (mean age: 74.6 ± 6.3) recruited from senior citizens' centres in Tokyo. The intervention group (n = 79) received a 3-month oral function promotion programme, which included facial muscle and tongue exercises and salivary gland massages. The control group (n = 62) did not receive this programme. Results: In the intervention group, the tongue coating scores decreased and the organoleptic score of oral malodour fell. The amount of food debris in the oral cavity decreased and the tongue dryness improved. Furthermore, the salivary flow rate increased. The length of time for maintaining the tongue in the forward position increased from 11.2 s to 18.7 s, and the number of times for moving the tip of the tongue in a clockwise circular motion, counter-clockwise circular motion and side-to-side motion within 30 s, increased from 14.5 to 20.6, 14.5 to 20.2, and 17.2 to 23.3 respectively. The number of times for movement of the lips significantly improved from 23.0 to 28.8 and the pronunciation of words was observed to be clearer. Conclusion: An oral function promotion programme was effective in improving the oral health status and oral function of an independent elderly population. © 2009 The Gerodontology Society and John Wiley & Sons A/S.
-
HUMAN & EXPERIMENTAL TOXICOLOGY 28(11) 703-713 2009年11月 査読有りPolybrominated diphenyl ethers (PBDEs) are used as flame retardants to prevent combustion in consumer products, such as electronics, construction materials, and textiles and, therefore, have become important commercial substances. PBDEs were also detected in maternal blood, breast milk, umbilical cord blood, and cord tissue, thereby indicating that fetuses were also exposed to PBDEs. The purpose of this study is to identify the effect of PBDEs on human umbilical vein endothelial cells (HUVECs). Cultured HUVECs were exposed to a commercial mixture of penta-BDE (DE71), octa-BDE (DE79), and deca-BDE (DE83). Each gene expression that was altered in DNA microarray was confirmed by real-time reverse transcription-polymerase chain reaction and Western blotting analysis. The results indicated that gene expressions concerning antioxidant system, i.e., thioredoxin family, 24-dehydrocholesterol reductase (DHCR24), and tumor suppressor protein p53, were altered by PBDEs exposure in HUVECs. Moreover, it was demonstrated that thioredoxin-interacting protein (TXNIP) was a target gene in exposure to DE71 and DE79 in HUVECs, by drastically decreasing time-dependent TXNIP expression in HUVECs.
-
TOXICOLOGY LETTERS 189 S254 2009年9月13日 査読有り
-
TOXICOLOGY LETTERS 189 S204 2009年9月13日 査読有り
-
ENDOCRINE JOURNAL 56(1) 131-139 2009年2月 査読有りPerinatal exposure to diethylstilbestrol (DES) can have numerous adverse effects on the reproductive organs later in life, Such as vaginal clear-cell adenocarcinoma. Epigenetic processes including DNA methylation may be involved in the mechanisms. We subcutaneously injected DES to neonatal C57BL/6 mice. At days 5, 14, and 30, expressions of DNA methyltransferases (Dnmts) Dnmt1, Dnmt3a, and Dnmt3b, and transcription factors Sp1 and Sp3 were examined. We also performed restriction landmark genomic scanning (RLGS) to detect aberrant DNA methylation. Real-time RTPCR revealed that expressions of Dnmt1, Dnmt3b, and Sp3 were decreased at day 5 in DES-treated mice, and that those of Dnmt1, Dnmt3a, and Sp1 were also decreased at day 14. RLGS analysis revealed that 5 genomic loci were demethylated, and 5 other loci were methylated by DES treatment. Two loci were cloned, and differential DNA methylation was quantified. Our results indicated that DES altered the expression levels of Dnmts and DNA methylation.
-
Anatomical Sciences Education 2(6) 273-279 2009年 査読有りAt Chiba University, gross anatomy laboratory sessions (" laboratories") are required for physical therapy students. Though most physical therapy schools require their students to participate in laboratories so that they will better understand the structure of the human body, few data exist on the value of these laboratories specifically for physical therapy students. We administered questionnaires to physical therapy undergraduate students both before and after they participated in laboratories. Questionnaire items focused on student attitudes toward the laboratories and on human life and dignity. Data from 83 students were analyzed, with the following results: (1) 74.7% of students had a positive attitude about attending laboratories before doing so (2) with few exceptions, students' attitudes about upcoming laboratories grew more positive after experiencing the laboratory work (P < 0.001) (3) laboratories caused students to contemplate the topics of human life and dignity and (4) 83.1% of students hoped to participate in laboratories at least four times. These results indicate that laboratories reinforce physical therapy students' positive attitudes about laboratory learning and promote student reflection on human life and dignity. This study provides support for the implementation of multiple laboratory sessions using cadavers into a uniform curriculum for physical therapy students in Japan. © 2009 American Association of Anatomists.
-
TOXICOLOGY AND APPLIED PHARMACOLOGY 234(1) 33-40 2009年1月 査読有りPreviously, we reported that 2,3.7,8-tetrachlorodibenzo-p-dioxin (TCDD) evoked developmental toxicity required activation of aryl hydrocarbon receptor type 2 (AHR2), using zebrafish embryos. However, the downstream molecular targets of AHR2 activation are largely unknown and are the focus of the present investigation. TCDD induces cyclooxygenase 2 (COX2), a rate-limiting enzyme for prostaglandin synthesis in certain cells. In the present study, we investigated the role of the COX2-thromboxane pathway in causing a specific endpoint of TCDD developmental toxicity in the zebrafish embryo, namely, a decrease in regional blood flow in the dorsal midbrain. it was found that the TCDD-induced reduction in mesencephalic vein blood flow was markedly inhibited by selective COX2 inhibitors, NS-398 and SC236, and by a general COX inhibitor, indomethacin, but not by a selective COX1 inhibitor, 5C-560. Gene knock-down of COX2 by two different types of morpholino antisense oligonucleotides, but not by their negative homologs, also protected the zebrafish embryos from mesencephalic vein circulation failure caused by TCDD. This inhibitory effect of TCDD oil regional blood flow in the dorsal midbrain was also blocked by selective antagonists of the thromboxane receptor (TP). Treatment of control zebrafish embryos with a TP agonist also caused a reduction in mesenceplialic vein blood flow and it too was blocked by a TP antagonist, without any effect on trunk circulation. Finally, gene knock-down of thromboxane A synthase 1 (TBXS) with morpholinos but not by the morpholinos' negative homologs provided significant protection against TCDD-induced mesencephalic Circulation failure. Taken together, these results point to a role of the prostanoid synthesis pathway via COX2-TBXS-TP in the local circulation failure induced by TCDD in the dorsal midbrain of the zebrafish embryo. (C) 2008 Elsevier Inc. All rights reserved.
-
CLEFT PALATE-CRANIOFACIAL JOURNAL 45(5) 545-551 2008年9月 査読有りObjectives: The aim of this study was to analyze epigenetic (specifically, DNA methylation) participation in the mechanisms of cleft palate only induced by maternal exposure to all-trans retinoic acid in mice. Design: Cleft palate only was induced in fetuses by maternal exposure to all-trans retinoic acid. Their secondary palates were excised for analysis. Cytosine extension assay and restriction landmark genomic scanning were performed to analyze DNA methylation status. The expression levels of the DNA methyltransferases were examined by real-time reverse transcriptase-polymerase chain reaction. Results: Using cytosine extension assay, on gestation day 14.5, the status of DNA methylation within CpG islands and in global DNA was decreased significantly in all-trans retinoic acid-treated groups compared with the controls (p < .01 and p < .05). In the controls, the status within CpG islands on gestation day 14.5 was significantly increased compared with gestation days 13.5 and 18.5 (p < .01). Using real-time reverse transcriptase-polymerase chain reaction, there was no significant change in the expression of DNA methyltransferases, except on gestation day 18.5. Using restriction landmark genomic scanning on gestation day 18.5, five spots (0.49%) in the controls and one spot (0.1%) in all-trans retinoic acid-treated groups were specifically detected. Conclusions: These results indicate that changes in DNA methylation may play an important role in the manifestation of cleft palate only caused by environmental factors such as maternal exposure to all-trans retinoic acid.
-
BIOLOGY OF REPRODUCTION 79(3) 537-545 2008年9月 査読有りDuring epididymal transit, sperm acquire the ability to initiate rapid forward progressive motility on release into the female reproductive tract or physiological media. Glycolysis is the primary source of the ATP necessary for this motility in the mouse, and several novel glycolytic enzymes have been identified that are localized to the principal piece region of the flagellum. One of these is the spermatogenic cell-specific type 1 hexokinase isozyme (HK1S), the only member of the hexokinase enzyme family detected in sperm. Hexokinase activity was found to be lower in immotile sperm immediately after removal from the cauda epididymis (quiescent) than in sperm incubated in physiological medium for 5 min and showing rapid forward progressive motility (activated). However, incubating sperm in medium containing diamide, an inhibitor of disulfide bond reduction, resulted in lower motility and HK activity than in controls. HK1S was present in dimer and monomer forms in extracts of quiescent sperm but mainly as a monomer in motile sperm. A dimer-size band detected in quiescent sperm with phosphotyrosine antibody was not detected in activated sperm, and the monomer-size band was enhanced. In addition, the general protein oxido-reductase thioredoxin-1 was able to catalyze the in vitro conversion of HK1S dimers to the monomeric form. These results strongly suggest that cleavage of disulfide bonds in HK1S dimers contributes to the increases in HK activity and motility that occur when mouse sperm become activated.
-
Chemosphere 73(1 Suppl) S235-8 2008年8月 査読有り
-
MOLECULAR REPRODUCTION AND DEVELOPMENT 75(4) 632-640 2008年4月 査読有りHexokinase is the first enzyme in the glycolytic pathway and utilizes ATP to convert glucose to glucose-6-phosphate (G6P). We previously identified three variant transcripts of Hk1 that are expressed specifically in spermatogenic cells, have different 5 ' untranslated regions, and encode a protein (HK1S, spermatogenic cell-specific type 1 hexokinase) in which the porin-binding domain (PBD) of HK1 is replaced by a novel N-terminal spermatogenic cell-specific region (SSR). However, the level of expression of the individual variant transcripts or of the other members of the hexokinase gene family (Hk2, Hk3, and Gck) in spermatogenic cells remains uncertain. We show that Hk1, Hk2, and Hk3 transcripts levels are quite low in spermatocytes and spermatids and Gck transcripts are relatively abundant in spermatids, but that glucokinase (GCK) is not detected in spermatozoa. Using real time RT-PCR (qPCR) with primers specific for each of the three variant forms and RNA from whole testis and isolated germ cells, we found that transcripts for Hk1_v2 and Hk1_v3, but not for Hk1_v1, are relatively high in spermatids. Similar results were seen using spermatogenic cells isolated by laser-capture microdissection (LCM). Immunoblotting studies found that HK1S is abundant in sperm, and immunostaining confirmed that HK1S is located mainly in the principal piece of the sperm flagellum, where other spermatogenic cell-specific glycolytic enzymes have been found. These results strongly suggest that HK1, HK2, HK3, and GCK are unlikely to have a role in glycolysis in sperm and that HK1S encoded by Hk1_v2 and Hk1_v3 serves this role.
-
Endocrine Journal 55(6) 1071-1084 2008年 査読有りBrominated flame retardants (BFRs) are used to prevent combustion in consumer products. Examples of BFRs are polybrominated diphenyl ethers (PBDEs), tetrabromobisphenol A (TBBPA), and tribromophenol (TBP). These compounds are reported to have adverse effects on human health and endocrine disrupting effects. The purpose of this study was to identify the Japanese perinatal exposure to PBDEs, hydroxylated PBDE metabolites (OH-PBDEs), TBBPA, and TBP compared with polychlorinated biphenyls (PCBs) and hydroxylated PCB metabolites (OH-PCBs). We investigated the concentrations of these compounds in maternal blood, maternal milk, cord blood, and umbilical cords from 16 Japanese mother-infant pairs by HRGC/HRMS. PBDEs were detected in all samples of maternal blood (mean ± SD median = 25 ± 23 pg/g 18 pg/g wet weight), maternal milk (140 ± 220 pg/g 59 pg/g wet weight), cord blood (4.8 ± 6.5 pg/g 1.6 pg/g wet weight), and umbilical cords (3.1 ± 3.1 pg/g 2.1 pg/g wet weight). The mothers were divided into two groups, a high-concentration group and a low-concentration group. The percentage of BDE-47 showed the greatest difference between the two groups. 6-OH-BDE-47, TBBPA, and TBP were detected in all umbilical cord samples (mean ± SD median = 8.4 ± 8.1 pg/g 8.0 pg/g, 16 ± 5.5 pg/g 15 pg/g, and 33 ± 8.2 pg/g 32 pg/g wet weight respectively), but not in all maternal blood or cord blood samples. These results indicate that OH-PBDEs, TBBPA, and TBP, in addition to PBDEs, PCBs, and OH-PCBs, pass through the blood-placenta barrier and are retained in the umbilical cord.
-
Japanese Journal of Allergology 57(7) 828-834 2008年 査読有り
-
Journal of Occupational Health 49(6) 499-503 2007年11月 査読有り
-
HUMAN & EXPERIMENTAL TOXICOLOGY 26(10) 811-816 2007年10月 査読有りPolychlorinated biphenyls (PCBs) are a group of persistent pollutants that are detected in maternal serum and umbilical cord, suggesting that fetal exposure also needs to be considered. The effects of dioxin-like PCB congeners 3,3',4,4'-tetrachlorobiphenyl (PCB77) and 3,3',4,4',5-pentachlorobiphenyl (PCB126) and a non-dioxin-like compound 2,2',4,4',5,5'-hexachlorobiphenyl (PCB153) on the expression of endothelial nitric oxide synthase (eNOS), known to maintain blood flow to the fetus, in human umbilical vein endothelial cells (HUVECs) were investigated. The mRNA levels of eNOS, aryl hydrocarbon receptor (AhR) and cytochrome P450 (CYP) 1A1 in cells treated with 5 mu M PCBs for 24 hours were analysed by real-time RT-PCR. Cells were also treated with alpha-naphthoflavone (alpha NF), an AhR antagonist or ICI 182780, an estrogen receptor (ER) antagonist, one hour prior to PCB exposure, to observe the effects of these receptors on eNOS modulation. Each PCB increased the eNOS mRNA level by 4.5-fold that was markedly inhibited by alpha NF. ERs were also suspected of altering eNOS levels because ICI 182780 treatment resulted in a decrease in the eNOS level. These results suggest that the eNOS mRNA expression increases due to the action of PCBs related to both AhR and ERs in HUVECs, and that maternal PCB exposure could influence fetal circulation.
-
REPRODUCTIVE TOXICOLOGY 23(4) 507-512 2007年6月 査読有りIn our previous study, the vagina] opening (VO) day of C57BL/6 mice was accelerated several days by chronic exposure to a 0.05% isoflavone (IF) fortified diet. The purpose of this study was to investigate whether the acceleration of VO by IF (1) has a critical window, (2) is modified by IF exposure combined with 17 beta-estradiol (E2), and (3) has any relation with gene expressions of estrogen-related receptors (ERRs). As a result, we determined that the critical window for the acceleration of VO was between 15 and 21 days postnatal. The combined effect of E2 and IF was thought to be additional in the acceleration of VO. The gene expression of ERR gamma was significantly decreased in vagina by IF. The reduction of ERR gamma may have two possible sequelae: disarrangement of vaginal development and high risk of vaginal cancer. In conclusion, IF exposure has a critical window for acceleration of VO and may have adverse effect on mouse vagina. (c) 2007 Elsevier Inc. All rights reserved.
-
Reproductive toxicology (Elmsford, N.Y.) 23(3) 283-289 2007年4月 査読有り
-
了徳寺大学研究紀要 1 133-140 2007年3月31日The human gross anatomy is one of the important courses for a co-medical worker, such as a physical therapist, a sports trainer or a nurse. Similarly, the students at a co-medical school should learn the human gross anatomy course. To improve understanding of the human body, the educational human dissection view is important to translate the human structure into some optical information. In the present study, the survey was performed on the students in the school of physical therapy who took part in the educational human dissection tour at Chiba University. The total number of answers was 88 and the reply percentage was 77.2% (68 persons ; male=50, female=18). The 92.6% of students had interest in the anatomy and felt that their understanding of the structure of a human body was improved. Further, the students who had participated in the dissection for several times showed more understanding of the views. The results suggested that the multiple experience of the human anatomical views isuseful for the co-medical students to improve the quality of their understanding ofthe human body.
-
REPRODUCTIVE TOXICOLOGY 23(2) 153-157 2007年2月 査読有りPreviously, we reported that decreased epididymal expression of CD59 and decay accelerating factor (DAF) genes may affect sperm motility and the acrosome reaction in rats treated long-term (28 days) with sulfasalazine. To investigate the early effects of sulfasalazine on the male reproductive tract, we presently examined sperm motility, the acrosome reaction, and gene expression in the testes and epididymides of rats treated with sulfasalazine for 1, 7 or 14 days. Reduced sperm motility and acrosome reactions were noted on day 7, however, there were no remarkable changes in testicular gene expression. On the other hand, attenuated epididymal gene expression of CD59 and DAF was observed as early as day 1. As CD59 and DAF are secreted from the epididymis and play a role in sperm maturation, we hypothesize that sulfasalazine affects sperm maturation as an early effect and that CD59 and DAF genes are related to the negative effect. (c) 2006 Elsevier Inc. All rights reserved.
-
アレルギー 56(8) 933-933 2007年
-
アレルギー 56(3) 266-266 2007年
-
JOURNAL OF HEALTH SCIENCE 52(6) 848-848 2006年12月 査読有り
-
Bioscience, biotechnology, and biochemistry 70(12) 2874-2882 2006年12月 査読有りIsoflavone (IF), a type of phytoestrogen, has multiple beneficial effects, but too much phytoestrogen can have adverse effects on offspring. To examine whether chronic exposure to high IF has adverse effects on reproductive development, mice offspring were exposed to IF through dietary administration to dams during pregnancy and lactation and to the offspring directly after weaning until sacrifice. In male offspring, there was no difference between the IF group and controls; however, in female offspring in the IF group, remarkably earlier puberty and induction of multioocyte follicles on postnatal day (PND) 21 were observed. Gene expression levels of estrogen receptor β decreased in the ovary and vagina on PND 21. These results suggest that chronic exposure to higher than normal levels of IF induces alterations in the reproductive development of female mice through an estrogenic effect.
-
REPRODUCTIVE TOXICOLOGY 22(4) 746-753 2006年11月 査読有りNeonatal administration of diethylstilbestrol (DES) to rodents has adverse effects on spermatogenesis. However, not many studies have been conducted to determine which type of cell - germ or somatic - is the major target of DES. In order to clarify this, we tried reciprocal germ cell transplantation-transplantation of germ cells from DES-treated mice into intact mice and germ cells from normal mice into DES-treated mice. The donor germ cells were tagged with the green fluorescent protein (GFP) gene in order to distinguish the exogenous germ cells from the endogenous cells. Moreover, to obtain a large number of spermatogonia from the testes of adult mice, we performed fractionation by centrifugation with Percoll. Consequently, we found that the germ cells collected from DES-treated mice have differentiated into normal sperms in normal seminiferous tubules. However, in the case of the transplantation of normal germ cells into the seminiferous tubules of DES-treated mice, defective spermatogenesis was observed. In conclusion, DES has adverse effects on the somatic cells that are involved in spermatogenesis rather than the germ cells. (c) 2006 Elsevier Inc. All rights reserved.
MISC
321-
千葉医学 100(3) 61-70 2024年6月type:text [要旨]千葉大学予防医学センターでは，国際連携の強化による，グローバル人材の育成や国際交流活動推進の一環として，2013年度より海外研修プログラムを開催している。2023年度は医学薬学府および看護学研究科の大学院生を主な対象として，9 月にスイス，ジュネーブ国連・国際機関訪問研修を実施し，11月には環境健康学についての集中講義をフランス，ニースおよびドイツ，ライプツィヒにて開催した。本稿では，フランス，コート・ダジュール大学にて行った千葉大学との共催集中講義およびニース市のプロジェクトチームによる環境健康都市政策セミナーについて報告する。3 日間にわたる集中講義は，各国から国際的な活躍をされている講師を迎え，コート・ダジュール大学医学部6年生の必須科目として認定された。同大学医学部学生，千葉大学大学院医学薬学府の大学院生ら総勢200名を超える現地での参加者を得て，英語で行われた。講義のメインテーマは予防医学で，6 つのセッションより構成された。具体的には，健康問題と環境要因に関する多くの観点からの講義とそれに続くディスカッションが活発に行われ，最終日には，ニース市の環境健康都市政策についてのセミナーが開催された。本プログラムが，環境健康学に関する理解と知見を深める契機となり，さらに，千葉大学が目指すグローバル人材の育成に貢献できることを期待する。 [ABSTRACT]The Center for Preventive Medical Sciences of Chiba University has produced overseas study programs since 2013, focusing on international collaboration in medical research and training students to play on a global stage. In 2023, we planned three overseas study programs, mainly for graduate students from the Graduate School of Medical and Pharmaceutical Sciences and Graduate School of Nursing, and held them in Switzerland, France, and Germany. This paper reports on the three-day intensive lecture program jointly organized by Chiba University and the University of Côte d’Azur, which was held in Nice, France, starting November 22nd. The lectures focused on environmental health, preventive medicine and featured international faculty from various countries. It was designated a compulsory subject for sixth-year medical students at the University of Côte d’Azur. Approximately 200 participants attended the lectures, including 180 medical students from the University of Côte d’Azur, 10 graduate students from Chiba University Graduate School of Medical and Pharmaceutical Sciences, and some medical residents of Côte d’Azur University Hospital. The intensive course consisted of six sessions, with various lectures as well as discussions on health issues and environmental factors. On the final day, the Nice City project team held a seminar on environmental and healthy city policies. A lively discussion took place during the program, with participants presenting and debating different perspectives. We aim for these programs to broaden participants’ perspectives and enhance their future opportunities.
所属学協会
7Works(作品等)
8共同研究・競争的資金等の研究課題
41-
日本学術振興会 科学研究費助成事業 2021年4月 - 2025年3月
-
日本学術振興会 科学研究費助成事業 2019年4月 - 2022年3月
-
日本学術振興会 科学研究費助成事業 2016年4月 - 2020年3月
-
日本学術振興会 科学研究費助成事業 2016年10月 - 2019年3月
-
日本学術振興会 科学研究費助成事業 2016年4月 - 2019年3月