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  2. 森 千里

森 千里

モリ チサト  (Chisato Mori)

基本情報

所属
千葉大学大学院 医学研究院　環境生命医学 教授 (医学博士)
学位
博士・乙(京都大学)
J-GLOBAL ID
200901071849698152
researchmap会員ID
1000026429
外部リンク
1984年旭川医科大学卒業、同年京都大学医学部助手。カナダマニトバ大学医学部客員講師、米国国立衛生研究所客員研究員、京都大学助教授を経て2000年に千葉大学医学部教授に就任。2001年より千葉大学大学院医学研究院環境生命医学教授。2008年より千葉大学予防医学センター長兼任。専門は、環境生命医学、発生学、解剖学。著書に「鷗外と脚気」（NTT出版）等。

研究キーワード

 4

研究分野

 2

経歴

 5

受賞

 3

論文

 335
  • 宮宗 秀伸, 深田 秀樹, 松野 義晴, 森 千里
    日本職業・災害医学会会誌 54(臨増) 別204-別204 2006年10月  
  • Kimihide Ohmichi, Masatoshi Komiyama, Yoshiharu Matsuno, Yuji Sawabe, Hidenobu Miyaso, Hideki Fukata, Masayoshi Ohmichi, Tomoko Kadota, Fumio Nomura, Chisato Mori
    Journal of Health Science 52(5) 642-647 2006年10月  査読有り
    In the gross anatomy laboratory, which is one of the compulsory subjects in most medical and dental schools, participants cannot avoid exposure to formaldehyde (FA), which is emitted from cadavers during dissection. FA has been recognized as a harmful chemical and we have previously reported that symptoms felt by participants in a gross anatomy laboratory are similar to those of allergic diseases. Although immunoglobulin E (IgE)-mediated sensitization to FA is a matter of controversy, it is possible that IgE production is evoked during a gross anatomy laboratory and is responsible for the reported symptoms. In order to test this hypothesis, we examined the relationships between the personal FA exposure levels and plasma IgE levels in a gross anatomy laboratory. In the laboratory, the personal FA exposure levels ranged from 0.33 to 1.47 ppm. Total blood IgE levels did not increase significantly and specific IgE to FA was negative during the laboratory sessions. Thus, from this study, we cannot support the hypothesis that the exposure to FA triggers an IgE-mediated reaction in this study. In conclusion, exposure to FA does not induce IgE production during gross anatomy laboratories at our school. <br>
  • Reiko Anahara, Yoshiro Toyama, Mamiko Maekawa, Masayuki Kai, Fumitoshi Ishino, Kiyotaka Toshimori, Chisato Mori
    FOOD AND CHEMICAL TOXICOLOGY 44(7) 1050-1056 2006年7月  査読有り
    Flutamide (FLUT) has potent anti-androgenic activity and is used in the medical field and in a screening test to detect endocrinologically active compounds. Our previous study demonstrated that FLUT induced histological deformation of spermatids and ultrastructural defects of the apical ectoplasmic specialization (ES) in the mouse testis. The apical ES is an actin-based junctional structure between the Sertoli cells and germ cells. Cortactin, an actin-binding protein, is found in the actin layer of ES. The protein level of cortactin was decreased in FLUT-treated testes as shown by Western blot analysis. The detailed analysis indicated that the protein level was drastically decreased in FLUT-treated seminiferous tubules of stages from VI to IX. Immunohistochemistry and immunoelectron microscopy showed that FLUT depressed cortactin expression in the apical ES. In addition, the effect of FLUT on cortactin localization appeared between 12 h and 8 days (about 180 h) after a one-day treatment. These results suggest that FLUT depressed the expression of cortactin in the apical ES with stage specificity. Therefore, the initial target of FLUT may be the cell-cell interactions between the Sertoli and germ cells. To our knowledge, this study is the first to document the decrease of cortactin expression in the abnormal apical ES following treatment with FLUT. (c) 2006 Elsevier Ltd. All rights reserved.
  • Anahara R, Toyama Y, Maekawa M, Yoshida M, Kai M, Ishino F, Toshimori K, Mori C
    Biochemical and biophysical research communications 346(1) 276-280 2006年7月  査読有り
  • Anahara R, Yoshida M, Toyama Y, Maekawa M, Kai M, Ishino F, Toshimori K, Mori C
    Archives of histology and cytology 69(2) 101-107 2006年6月  査読有り
  • 川城 由紀子, 宮宗 秀伸, 穴原 玲子, 松野 義晴, 森 千里
    解剖学雑誌 81(Suppl.) 158-158 2006年3月  
  • 宮宗 秀伸, 門田 朋子, 松野 義晴, 小宮山 政敏, 太田 昌彦, 穴原 玲子, 高島 杏佳, 川城 由紀子, 森 千里
    解剖学雑誌 81(Suppl.) 158-158 2006年3月  
  • Kimihide Ohmichi, Masatoshi Komiyama, Yoshiharu Matsuno, Yoshimitsu Takanashi, Hiroshi Miyamoto, Tomoko Kadota, Mamiko Maekawa, Yoshiro Toyama, Yukitoshi Tatsugi, Toshihiko Kohno, Masayoshi Ohmichi, Chisato Mori
    Environmental Science and Pollution Research International 13 120-124 2006年3月  査読有り
  • R Anahara, Y Toyama, M Koda, S Honma, J Nishihira, K Toshimori, C Mori
    REPRODUCTIVE TOXICOLOGY 21(2) 167-170 2006年2月  査読有り
    Macrophage migration inhibitory factor (MIF) is a multifunctional protein that acts as a pro-inflammatory cytokine, pituitary hormone, immunoregulator and mitogen. The MIF gene knockout (MIFKO) mouse has been used to study the MIF response in many tissues, such as with skin injury and spinal cord injury; however, there is little information about the MIFKO mouse testis. This study reports the levels of serum and intratesticular estradiol and testosterone, the ultrastructure of the testis, and preliminary findings from in vitro fertilization. Our results revealed a decrease in estradiol and testosterone levels and deformation in spermiogenesis, in the MIFKO mouse. These initial findings study may lead to a better understanding of the role that MIF plays in the mouse testis. (c) 2005 Elsevier Inc. All rights reserved.
  • 深田 秀樹, 井上 万里子, 大森 直子, 川城 由紀子, 小川 俊子, 戸高 恵美子, 森 千里
    環境ホルモン学会研究発表会要旨集 8回 86-86 2005年9月  査読有り
  • 小川 俊子, 戸高 恵美子, 川城 由紀子, 深田 秀樹, 森 千里
    環境ホルモン学会研究発表会要旨集 8回 312-312 2005年9月  査読有り
  • Fukushima Tamio, Yamamoto Toshinori, Kikkawa Rie, HAMADA Yoshimasa, KOMIYAMA Masatoshi, MORI Chisato, HORII Ikuo
    The Journal of Toxicological Sciences 30(3) 195-206 2005年8月29日  
    Predictive biomarkers of testicular toxicity are needed for an efficient development of drugs. The purpose of the present study was to obtain further insight into the toxicity mechanisms of various male reproductive toxicants and to detect genomic biomarkers for rapid screening of testicular toxicity. Four reproductive toxicants, 2, 5-hexanedione (Sertoli cells toxicant), ethylene glycol monomethyl ether (EGME; spermatocytes toxicant), cyclophosphamide (spermatogonia toxicant) and sulfasalazine, were orally administered to male rats once. Six hours after the single dosing, gene expression in the testes was monitored by cDNA microarray and real-time RT-PCR and the testes were histopathologically examined. No histopathological abnormality was detected except for slight degeneration of spermatocytes in the EGME-treated testes. cDNA microarray analysis revealed differential gene expression profiles, and it was possible based on the profiles to characterize the action of the compounds in the testes. Interestingly, 3 spermatogenesis-related genes - heat shock protein 70-2, insulin growth factor binding protein 3 and glutathione S transferase pi - were affected by all the compounds. The above changes of gene expression were detectable within a short period after the dosing prior to the appearance of obvious pathological changes. These data suggest that cDNA microarray is a useful technique for evaluation of primary testicular toxicity. Furthermore, we propose the above 3 spermatogenesis-related genes as potential biomarkers of testicular toxicity.
  • Masatoshi Komiyama, Chisato Mori
    Handbook of Toxicogenomics: A Strategic View of Current Research and Applications 487-506 2005年5月23日  査読有り
    Toxicogenomic analysis of human umbilical cords revealed that the hierarchical order of the umbilical cords clustered according to their gene expression profiles corresponded to their order according to their total concentrations of chemicals, with one exception. In the exceptional umbilical cord, the total concentration of chemicals was lowest, but its gene expression profile was most similar to that of the umbilical cord exhibiting the highest level of total chemical concentrations. These results suggest that gene expression in umbilical cords may reflect their exposure to some persistent chemicals. In addition, the expression profile may reflect contamination with a mixture of chemicals rather than that with each single compound. Furthermore an important suggestion from this study is that toxicogenomic analysis can detect potential high-risk groups, because genetically higher susceptibility as well as higher exposure of an individual to multiple chemicals can be regarded as a higher health risk to the individual.
  • T Fukushima, M Kato, T Adachi, Y Hamada, M Horimoto, M Komiyama, C Mori, Horii, I
    TOXICOLOGICAL SCIENCES 85(1) 675-682 2005年5月  査読有り
    Sulfasalazine (SASP) has been reported to depress the fertility in men and experimental male animals, but the fundamental mechanisms of infertility caused by SASP are still unknown. This study was designed to investigate the mechanisms of infertility in rats treated with SASP at a dose of 600 mg/kg for 28 days, including monitoring of sperm motility using computer associated sperm analysis system and acrosome reaction by FITC-concanavalin A lectin staining. The sperm motility and acrosome reaction, which are important for fertilization, were significantly reduced by SASP. Furthermore, to investigate the molecular mechanisms of infertility induced by SASP, mRNA expression analysis in the testes was performed using cDNA microarray as a first screening. It was revealed that CD59, which is located on the acrosomal membrane and is known to be important for the reproductive function of sperm, was affected in the testes; this was also confirmed by real-time PCR analysis, but the spermatogenesis-related genes examined in this study were not affected. Therefore, we focused on CD59 and two other acrosome membrane related-genes: MCP and DAF. CD59, MCP, and DAF in the epididymides of SASP-treated rats were significantly decreased as assessed by real-time RT-PCR analysis and additionally, the expression of CD59 protein was found to be decreased by Western blotting. These results allowed us to hypothesize that the suppression of epididymal acrosomal membrane proteins synthesis with their consequent reduced incorporation to the sperm membrane leads to a depressed sperm motility and acrosome reaction, and thereby leads to infertility in SASP treated male rats.
  • T Adachi, Y Okuno, S Takenaka, K Matsuda, N Ohta, K Takashima, K Yamazaki, D Nishimura, K Miyatake, C Mori, G Tsujimoto
    FOOD AND CHEMICAL TOXICOLOGY 43(4) 529-535 2005年4月  査読有り
    In this study, we examined the effects of exposure to phytoestrogen (daidzein), 17beta-estradiol (E(2)) diethylstilbestrol (DES) and staurosporin on the TM4 testicular cell line, using comprehensive analysis, such as cDNA microarray and two-dimension polyacrylamide gel electropholesis (2D-PAGE) analysis, and we demonstrated if these toxicogenomic analyses could classify the chemical compounds. First, RNA was extracted from TM4 cells that had been treated with daidzein (80 muM), DES, E(2) (40 muM) and stauroporin (100 nM) for 30 min. We performed cDNA microarray analysis, and the expression ratio data thus obtained were then analyzed using hierarchical clustering. This hierarchical clustering showed that daidzein exposure induced a different effect on gene expression change from that of E(2), DES and staurosporin. Next, protein extracted from TM4 cells also underwent cDNA microarray analysis for 3 h. We performed 2D-PAGE analysis, and the spot intensity ratio data thus obtained were analyzed using hierarchical clustering. As with cDNA microarray, the hierarchical clustering of protein spot ratios showed that daidzein exposure induced a different effect on gene expression change from that of the other substances. In conclusion, we have demonstrated for the first time that classification of these chemicals can be performed by clustering analysis, using data from cDNA microarray and 2D-PAGE analyses, and that exposure to daidzein induces effects different from those of E2, DES and staurosporin. (C) 2005 Elsevier Ltd. All rights reserved.
  • Tabuchi Y, Toyama Y, Toshimori K, Komiyama M, Mori C, Kondo T
    Biochemical and biophysical research communications 329(3) 812-823 2005年4月  査読有り
  • Todaka E, Sakurai K, Mori C
    Reproductive medicine and biology 4(1) 65-70 2005年3月  査読有り
  • M Hashimoto, M Koda, H Ino, K Yoshinaga, A Murata, M Yamazaki, K Kojima, K Chiba, C Mori, H Moriya
    ACTA NEUROPATHOLOGICA 109(2) 165-180 2005年2月  査読有り
    The purpose of this study was to use a cDNA microarray to identify new genes involved in healing of spinal cord injury. C57BL/6 mice (7-8 weeks, male) were subjected to spinal cord compression injury (SCI) at the T7/8 level (20 g, 5 min; SCI group). For the control group, mice underwent only laminectomy. Mice were killed at 1, 3 and 7 days. cDNA transcribed from mRNA was hybridized to NIA mice 15K microarrays at each time point. We found 84 genes showing significant expressional changes, including higher and lower expression levels in the SCI groups than in the control [more than 1.0 or less than -1.0 using log ratio (base 2)]. Five genes were selected for further quantitative gene expression analysis by real-time reverse transcription (RT)-PCR. For histological examination, we applied in situ hybridization and fluorescence immunohistochemistry. Cathepsin D, metallothionein-1 (MT-1), metallothionein-2 (MT-2), osteopontin (OPN), and tenascin-C were selected for quantitative and histological analysis. Microarray analysis revealed that SCI led to the up-regulation of OPN and cathepsin D expression at 7 days and also of MT-1, MT-2, and tenascin-C expression at 1 day. Tenascin-C was re-up-regulated at 7 days. These values agreed with those of real-time RT-PCR analysis. By double labeling with in situ hybridization and fluorescence immunohistochemistry, MT-1, MT-2 and tenascin-C expression was observed in neurons and glial cells at 1 day, whereas at 7 days the main MT-2 and tenascin-C expression was found in fibronectin-positive fibroblasts. The main cathepsin D and OPN expression was observed in activated macrophages/microglia at 3 and 7 days. The five genes picked up by microarray gene expression profiling were shown to exhibit temporal and spatial changes of expression after SCI. This system is potentially useful for identifying genes that are involved in the response to SCI.
  • K Kijima, K Toyosawa, M Yasuba, N Matsuoka, T Adachi, M Komiyama, C Mori
    TOXICOLOGY AND APPLIED PHARMACOLOGY 200(2) 103-110 2004年10月  査読有り
    To investigate the effects of di(2-ethylhexyl) phthalate (DEHP) on gene expression in rat testis, 6-week-old male Sprague-Dawley rats were given a single oral dose of 20 or 2000 mg/kg and euthanized 3, 6, 24, or 72 It thereafter. Terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL)-positive cells were significantly increased in the testis at 24 and 72 h after the exposure to 2000 mg/kg of DEHP. On cDNA microarray analysis, in addition to apoptosis-related genes, genes associated with atrophy, APEX nuclease, MutS homologue (E. coli), testosterone-repressed-prostatic-message-2 (TRPM-2), connective tissue growth factor, collagen alpha 2 type V, and cell adhesion kinase were differentially expressed. To investigate the relationship between histopathological alteration and gene expression, we selected genes associated with apoptosis and analyzed their expression by real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR). With 20 mg/kg of DEHP treatment, bcl-2, key gene related to apoptosis, was increased. Up-regulation of bcl-2, inhibitor of Apaf-1/caspase-9/caspase-2 cascade of apoptosis, may be related to the fact that no morphological apoptotic change was induced after dosing of 20 mg/kg DEHP. With 2000 mg/kg of DEHP treatment, the apoptotic activator cascade, Fas/FasL, FADD/caspase-8/caspase-3 cascade, and Apaf-1/caspase-9/caspase-2 cascade were increased and bcl-2 was decreased. Thus, these gene regulations might lead the cells into apoptosis in the case of high exposure to DEHP. In contrast, FADD/caspase-10/caspase-6 cascade and caspase-11/caspase-3 cascade were not increased. These results indicate that the cascades of FADD/caspase-10/caspase-6 and caspase-11/caspase-3 are not related to apoptosis with DEHP treatment. (C) 2004 Elsevier Inc. All rights reserved.
  • Sakurai K, Todaka E, Saito Y, Mori C
    Internal medicine (Tokyo, Japan) 43(9) 792-795 2004年9月  査読有り
  • KB Koh, M Komiyama, Y Toyama, T Adachi, C Mori
    ASIAN JOURNAL OF ANDROLOGY 6(2) 93-98 2004年6月  査読有り
    Aim: To isolate and transplant germ cells from adult mouse testes for transplantation. Methods: In order to distinguish transplanted cells from endogenous cells of recipients, donor transgenic mice expressing green fluorescent protein (GFP) were used. Germ cells were collected from the donors at 10-12 weeks of age and spermatogonia were concentrated by percoll fractionation and transplanted into recipient seminiferous tubules that had been previously treated with busulfan at 5 weeks of age to remove the endogenous spermatogenic cells. Results: Twenty weeks after the transplantation, a wide spread GFP signal was observed in the recipient seminiferous tubules. The presence of spermatogenesis and spermatozoa was confirmed in sections of 12 out of 14 testes transplanted (86%). However, when germ cells were transplanted without concentration the success rate was zero (0/9). Conclusion: Germ cells from adult mouse testes can be successfully transplanted into recipient seminiferous tubules if the cell population is rich in spermatogonia and the percoll fractionation is useful in obtaining such a cell population.
  • Sakurai K, Todaka E, Suzuki Y, Mori C
    Congenital anomalies 42(4) 323-326 2002年12月  査読有り
  • T Tobe, N Toyota, Y Matsuno, M Komiyama, T Adachi, H Ito, C Mori
    ARCHIVES OF HISTOLOGY AND CYTOLOGY 65(3) 279-290 2002年8月  査読有り
    We examined the immunolocalization of isoforms of muscle proteins, myosin and troponin, in the cremaster muscle of the undescended testis (cryptorchidism). In cryptorchid rats induced by a nonsteroidal androgen antagonist, flutamide, the cremaster muscle contained embryonic myosin and embryonic/cardiac troponin T in both immunofluorescence microscopy and Western blotting using antibodies against myosin and troponin T specific for embryonic, cardiac and fast skeletal muscles. However, in muscles other than the cremaster muscle, i.e., the masseter, pectoral and abdominal muscles, embryonic isoforms of these proteins were undetectable by immunohistochemistry with these antibodies, even in the muscles from cryptorchid rats. Our results showing that high levels of embryonic isoforms of muscle proteins were specifically present in the cremaster muscle of cryptorchid rats induced by flutamide suggest that flutamide treatment of pregnant rats might affect genes controlling the development of the lumbar region of the fetus body resulting in the presence of embryonic protein isoforms in the cremaster muscles which are closely associated with undescended testes.
  • Mori C, Hamamatsu A, Fukata H, Koh KB, Nakamura N, Takeichi S, Kusakabe T, Saito T, Morita M, Tanihara S, Kayama F, Shiyomi M, Yoshimura J, Sagisaka K
    Anatomical science international 77 109-116 2002年6月  査読有り
  • Y Uehara, C Mori, T Noda, K Shiota, N Kitamura
    GENESIS 27(3) 99-103 2000年7月  査読有り
    While the targeted disruption of a gene is a powerful tool for investigating the physiological functions of that gene, disruption of a gene essential for embryogenesis leads to embryonic death. Rescue of the defect(s) causing embryonic death should promote survival, thus permitting further evaluation of the roles that the gene plays later in the developmental process. Disruption of the gene for mouse hepatocyte growth factor/scatter factor (HGF/SF) leads to middle-stage embryonic lethality because of a defect in placental development. Here we report that a single injection of HGF/SF at embryonic day 9.5 (E9.5) into the amniotic cavity of HGF/SF-/- embryos rescued the placental defect and resulted in the survival of the embryos until term. Histological analysis suggested that HGF/SF is also required at the late stage of development for tissue organogenesis. Thus, injection of a secreted factor can be a useful method to rescue the defects causing embryonic lethality. (C) 2000 Wiley-Liss, Inc.
  • Yoshihiko Uehara, Chisato Mori, Tetsuo Noda, Kohei Shiota, Naomi Kitamura
    Genesis 27(3) 124-135 2000年  査読有り
    The molecular pathways leading from indifferent mammalian gonad to either testis or ovary are not well understood. A number of genes, including the Y-linked sex determining gene SRY, have been shown to play roles in sex determination or differentiation, but there are clearly many missing elements to be found. We used suppression-subtractive hybridization to construct normalized cDNA libraries enriched for male-specific or female-specific transcripts in mouse fetal gonads. We describe the strategy used to efficiently screen these libraries for candidate sex-determination and gonado-genesis genes. One gene arising from these screens is vanin-1, which encodes a protein implicated in the induction of cell migration into the thymus. We find that vanin-1 is expressed male-specifically in Sertoli cells of the developing testis and may be involved in inducing cell migration from the adjacent mesonephros, a process known to be critical for testis development. This screening approach is likely to be applicable to the isolation and study of genes involved in a variety of developmental systems. (C) 2000 Wiley-Liss, Inc.
  • 森 千里
    産業衛生学雑誌 41 102-103 1999年  
  • H Naitoh, C Mori, Y Nishimura, K Shiota
    JOURNAL OF CRANIOFACIAL GENETICS AND DEVELOPMENTAL BIOLOGY 18(4) 202-210 1998年10月  査読有り
    Retinoic acid (RA) is mandatory for various biological processes and normal embryonic development but is teratogenic at high concentrations. In rodents, one of the major malformations induced by RA is cleft palate (CP). RA mediates its effects by RA receptors (RARs), but the expression patterns of RARs in the developing palate are still unclear. We investigated the normal expression of RAR alpha, beta, and gamma messenger RNAs (mRNAs) in the fetal mouse secondary palate and the effects of all-trans and 13-cis RAs on the expression of RAR mRNAs by Northern blot analysis. RAR alpha (2.8, 3.8 kb), RAR beta (3.3 kb), and RAR gamma (3.7 kb) mRNAs were detected in the fetal palate on gestational days (GD) 12.5-14.5. The expression of RAR alpha and gamma mRNAs did not show apparent sequential changes, but that of RAR beta mRNA increased at GD 13.5. Treatment of pregnant mice with 100 mg/kg all-trans RA induced CP in 94% of the fetuses and elevated the levels of RAR beta and gamma mRNAs in the fetal palate. The up-regulation of RAR beta mRNA by all-trans RA was more marked than that of RAR gamma mRNA. Treatment with 100 mg/kg 13-cis RA induced CP in only 19% of the fetuses. Although 13-cis RA elevated the RAR beta and gamma mRNA levels in fetal palates, its up-regulation was slower and less marked than that induced by all-trans RA. These findings indicate that the induction of RAR beta mRNA in the fetal palate correlates well with the tissue concentration of all-trans RA after RA treatment, and RAR beta may be one of the most influential candidate molecules for RA-induced teratogenesis.
  • DJ Dix, JW Allen, BW Collins, P Poorman-Allen, C Mori, DR Blizard, PR Brown, EH Goulding, BD Strong, EM Eddy
    DEVELOPMENT 124(22) 4595-4603 1997年11月  査読有り
    Spermatogenic cells synthesize a unique 70-kDa heat shock protein (HSP70-2) during prophase of meiosis I, and targeted disruption of the Hsp 70-2 gene has shown that this protein is required for spermatogenic cell differentiation in adult mice, HSP70-2 is associated with synaptonemal complexes formed between paired homologous chromosomes during meiotic prephase, The present study focuses on the nearly synchronous first wave of spermatogenesis in 12- to 28-day old juvenile mice to determine more precisely when HSP70-2 is required and what meiotic processes are affected by its absence. Spermatogenesis in homozygous mutant mice (Hsp70-2(-/-)) proceeded normally until day 15 when increasing numbers of pachytene spermatocytes became apoptotic and differentiation of cells beyond the pachytene stage began to falter, Synaptonemal complexes assembled in Hsp 70-2(-/-) mice and spermatocytes developed through the final pachytene substage, However, synaptonemal complexes failed to desynapse and normal diplotene spermatocytes were not observed, Metaphase spermatocytes were not seen in tissue sections from testes of Hsp70-2(-/-) mice, and expression of mRNAs and antigens characteristic of late pachytene spermatocytes (e.g., cyclin Al) and development of spermatids did not occur. Thus, HSP70-2 is required for synaptonemal complex desynapsis, and its absence severely impairs the transition of spermatogenic cells through the late meiotic stages and results in apoptosis beginning with the first wave of germ cell development in juvenile mice.
  • H Kaji, JL Platt, DER Sutherland, K Inoue, C Mori, K Shiota, M Imamura
    TRANSPLANT IMMUNOLOGY 5(1) 70-72 1997年3月  査読有り
  • C Mori, M Nakazawa
    JOURNAL OF THE ATOMIC ENERGY SOCIETY OF JAPAN 38(1) 33-38 1996年1月  査読有り
  • N NAKAMURA, C MORI, M NARITA, C UWABE, K SHIOTA
    ACTA HISTOCHEMICA ET CYTOCHEMICA 27(1) 81-87 1994年  査読有り
    We describe a rapid fluorescent in situ hybridization (FISH) procedure with X and Y chromosome-specific DNA probes for sexing human embryos and fetuses using paraffin-embedded tissues. The effects of various fixatives and pretreatment conditions on FISH efficiency were examined in different embryonic and fetal tissues. Fluorescent hybridization signals were identified in tissues which had been fixed in 4% paraformaldehyde (PFA) or 10% formalin but not in Bouin-fixed tissues. The preservation in 4% PFA at 4 degrees C yielded better FISH results than that in 10% formalin at room temperature. Another critical factor for visualization of fluorescent hybridization signals in paraffin sections was the duration of proteinase K digestion. Optimal digestion visualized hybridization signals more clearly probably because it increased the accessibility of DNA probes into nuclear DNA in paraffin sections. We have succeeded in shortening the hybridization time significantly and eliminating an immunocytochemical step for amplifying hybridization signals, and it has become possible to sex human conceptuses in paraffin sections within 5 hr. This FISH technique should be useful to sex fixed human embryos and fetuses retrospectively and to diagnose sex chromosome aneuploidies using fixed tissues.
  • EM EDDY, DA OBRIEN, JE WELCH, C MORI, MO ROSARIO, KD FULCHER
    UNDERSTANDING MALE INFERTILITY : BASIC AND CLINICAL APPROACHES 98 171-182 1993年  査読有り
  • S. Higashide, K. Inoue, H. Minote, T. Tobe, C. Mori, K. Shiota
    Archiv fur Japanische Chirurgie 61(4) 311-319 1992年  査読有り
  • Suzuki, N, Nakaoka, H, Hanazato, M, Nakayama, Y, Takaya, K, Mori, C, Emission rates of, substances from low-volatile-organic-compoun, paints. International Journal of Environmental Science, Technology
      査読有り

MISC

 321
  • 頓名 幸, 戸髙 恵美子, 坂部 貢, 山本 緑, 佐藤 圭吾, 森 千里
    千葉医学 100(3) 61-70 2024年6月  
    type:text [要旨]千葉大学予防医学センターでは，国際連携の強化による，グローバル人材の育成や国際交流活動推進の一環として，2013年度より海外研修プログラムを開催している。2023年度は医学薬学府および看護学研究科の大学院生を主な対象として，9 月にスイス，ジュネーブ国連・国際機関訪問研修を実施し，11月には環境健康学についての集中講義をフランス，ニースおよびドイツ，ライプツィヒにて開催した。本稿では，フランス，コート・ダジュール大学にて行った千葉大学との共催集中講義およびニース市のプロジェクトチームによる環境健康都市政策セミナーについて報告する。3 日間にわたる集中講義は，各国から国際的な活躍をされている講師を迎え，コート・ダジュール大学医学部6年生の必須科目として認定された。同大学医学部学生，千葉大学大学院医学薬学府の大学院生ら総勢200名を超える現地での参加者を得て，英語で行われた。講義のメインテーマは予防医学で，6 つのセッションより構成された。具体的には，健康問題と環境要因に関する多くの観点からの講義とそれに続くディスカッションが活発に行われ，最終日には，ニース市の環境健康都市政策についてのセミナーが開催された。本プログラムが，環境健康学に関する理解と知見を深める契機となり，さらに，千葉大学が目指すグローバル人材の育成に貢献できることを期待する。 [ABSTRACT]The Center for Preventive Medical Sciences of Chiba University has produced overseas study programs since 2013, focusing on international collaboration in medical research and training students to play on a global stage. In 2023, we planned three overseas study programs, mainly for graduate students from the Graduate School of Medical and Pharmaceutical Sciences and Graduate School of Nursing, and held them in Switzerland, France, and Germany. This paper reports on the three-day intensive lecture program jointly organized by Chiba University and the University of Côte d’Azur, which was held in Nice, France, starting November 22nd. The lectures focused on environmental health, preventive medicine and featured international faculty from various countries. It was designated a compulsory subject for sixth-year medical students at the University of Côte d’Azur. Approximately 200 participants attended the lectures, including 180 medical students from the University of Côte d’Azur, 10 graduate students from Chiba University Graduate School of Medical and Pharmaceutical Sciences, and some medical residents of Côte d’Azur University Hospital. The intensive course consisted of six sessions, with various lectures as well as discussions on health issues and environmental factors. On the final day, the Nice City project team held a seminar on environmental and healthy city policies. A lively discussion took place during the program, with participants presenting and debating different perspectives. We aim for these programs to broaden participants’ perspectives and enhance their future opportunities.
  • 高口倖暉, 江口哲史, 嶋谷圭一, 中岡宏子, 依田(津村)佳余, 中山誠健, 森千里, 森千里, 鈴木規道
    環境化学討論会要旨集(CD-ROM) 32nd 2024年  
  • 江口哲史, 高口倖暉, 川島孝行, 中岡宏子, 津村佳余, 嶋谷圭一, 中山誠健, 森千里, 鈴木規道
    環境化学討論会要旨集(CD-ROM) 32nd 2024年  
  • 嶋谷圭一, 高口倖暉, 津村佳余, 中山誠健, 松下尚史, 石坂閣啓, 川嶋文人, 森千里, 森千里, 鈴木規道
    室内環境学会学術大会講演要旨集(Web) 2023 2023年  
  • 津村佳余, 高口倖暉, 嶋谷圭一, 中山誠健, 森千里, 鈴木規道
    室内環境学会学術大会講演要旨集(Web) 2023 2023年  
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