医学部附属病院
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竹内 公一

タケウチ コウイチ  (Koichi Takeuchi)

基本情報

所属
千葉大学 医学部附属病院 特任准教授
学位
博士(医学)(自治医科大学(JMU))
公衆衛生学修士（専門職）(東京大学)
J-GLOBAL ID
200901015685377646
researchmap会員ID
1000291547

研究キーワード

 6

研究分野

 5

経歴

 2

学歴

 3

論文

 11
  • Tatsuo Kawarasaki, Kazuhiko Uchiyama, Atsushi Hirao, Sadahiro Azuma, Masayoshi Otake, Masatoshi Shibata, Seiko Tsuchiya, Shin Enosawa, Koichi Takeuchi, Kenjiro Konno, Yoji Hakamata, Hiroyuki Yoshino, Takuya Wakai, Shigeo Ookawara, Hozumi Tanaka, Eiji Kobayashi, Takashi Murakami
    JOURNAL OF BIOMEDICAL OPTICS 14(5) 054017 2009年9月  査読有り
    Animal imaging sources have become an indispensable material for biological sciences. Specifically, gene-encoded biological probes serve as stable and high-performance tools to visualize cellular fate in living animals. We use a somatic cell cloning technique to create new green fluorescent protein (GFP)-expressing Jinhua pigs with a miniature body size, and characterized the expression profile in various tissues/organs and ex vivo culture conditions. The born GFP-transgenic pig demonstrate an organ/tissue-dependent expression pattern. Strong GFP expression is observed in the skeletal muscle, pancreas, heart, and kidney. Regarding cellular levels, bone-marrow-derived mesenchymal stromal cells, hepatocytes, and islet cells of the pancreas also show sufficient expression with the unique pattern. Moreover, the cloned pigs demonstrate normal growth and fertility, and the introduced GFP gene is stably transmitted to pigs in subsequent generations. The new GFP-expressing Jinhua pigs may be used as new cellular/tissue light resources for biological imaging in preclinical research fields such as tissue engineering, experimental regenerative medicine, and transplantation. (C) 2009 Society of Photo-Optical Instrumentation Engineers. [DOI: 10.1117/1.3241985]
  • Nomoto, T, Okada, T, Shimazaki, K, Yoshioka, T, Nonaka-Sarukawa, M, Ito, T, Takeuchi, K, Katsura, K.I, Mizukami, H, Kume, A, Ookawara, S, Ikeda, U, Katayama, Y, Ozawa, K
    Gene Ther 16 383-391 2009年  査読有り
  • YH Liu, T Okada, K Sheykholeslami, K Shimazaki, T Nomoto, SI Muramatsu, T Kanazawa, K Takeuchi, R Ajalli, H Mizukami, A Kume, K Ichimura, K Ozawa
    MOLECULAR THERAPY 12(4) 725-733 2005年10月  査読有り
    Recombinant adeno-associated virus (AAV) vectors are of interest for cochlear gene therapy because of their ability to mediate the efficient transfer and long-term stable expression of therapeutic genes in a wide variety of postmitotic tissues with minimal vector-related cytotoxicity. In the present study, seven AAV serotypes (AAV1-5, 7, 8) were used to construct vectors. The expression of EGFP by the chicken P-actin promoter associated with the cytomegalovirus immediate-early enhancer in cochlear cells showed that each of these serotypes successfully targets distinct cochlear cell types. In contrast to the other serotypes, the AAV3 vector specifically transduced cochlear inner hair cells with high efficiency in vivo, while the AAV1, 2, 5, 7, and 8 vectors also transduced these and other cell types, including spiral ganglion and spiral ligament cells. There was no loss of cochlear function with respect to evoked auditory brain-stem responses over the range of frequencies tested after the injection of AAV vectors. These findings are of value for further molecular studies of cochlear inner hair cells and for gene replacement strategies to correct recessive genetic hearing loss due to monogenic mutations in these cells.
  • J Fujishiro, SI Takeda, Y Takeno, K Takeuchi, Y Ogata, M Takahashi, Y Hakamata, T Kaneko, T Murakami, T Okada, K Ozawa, K Hashizume, E Kobayashi
    NEPHROLOGY DIALYSIS TRANSPLANTATION 20(7) 1385-1391 2005年7月  査読有り
    Background. The characteristics of adenovirus-mediated gene transfer into the kidney are not well examined. We studied the effects of contact time and temperature on adenovirus-mediated transgene expression in rat kidneys, using catheter-based in vivo gene transfer and a rat renal transplant model ex vivo. Methods. An adenovirus vector containing the luciferase (Ad-Luc) or beta-galactosidase (Ad-LacZ) gene was introduced in vivo into the kidney via a renal artery catheter. Various contact times and temperatures were evaluated. Ex vivo, the renal graft was injected with Ad-Luc through the renal artery, chilled for 60 min and then transplanted. Luciferase expression was evaluated periodically by a non-invasive bioimaging system or histology. Cells expressing the LacZ gene were identified by immunoelectron microscopy. Results. In in vivo gene transfer, successful transgene expression was achieved; however, its efficiency was independent of contact time or temperature. In ex vivo gene transfer, transgene expression in the renal graft peaked early and gradually decreased. Strong gene expression was observed in the recipients' livers. LacZ expression was detected in fibroblasts, parietal epithelial cells of Bowman's capsule, mesangial cells, podocytes and tubular cells. Conclusions. This study generated new information about in vivo and ex vivo gene transfer into the kidney, which would be useful for renal gene therapy.
  • Toru Yoshioka, Naohide Ageyama, Hiroaki Shibata, Takanori Yasu, Yoshio Misawa, Koichi Takeuchi, Keiji Matsui, Keiji Yamamoto, Keiji Terao, Kazuyuki Shimada, Uichi Ikeda, Keiya Ozawa, Yutaka Hanazono
    Stem cells (Dayton, Ohio) 23(3) 355-64 2005年3月  査読有り
    Rodent and human clinical studies have shown that transplantation of bone marrow stem cells to the ischemic myocardium results in improved cardiac function. In this study, cynomolgus monkey acute myocardial infarction was generated by ligating the left anterior descending artery, and autologous CD34(+) cells were transplanted to the peri-ischemic zone. To track the in vivo fate of transplanted cells, CD34(+) cells were genetically marked with green fluorescent protein (GFP) using a lentivirus vector before transplantation (marking efficiency, 41% on average). The group receiving cells (n = 4) demonstrated improved regional blood flow and cardiac function compared with the saline-treated group (n =4) at 2 weeks after transplant. However, very few transplanted cell-derived, GFP-positive cells were found incorporated into the vascular structure, and GFP-positive cardiomyocytes were not detected in the repaired tissue. On the other hand, cultured CD34(+) cells were found to secrete vascular endothelial growth factor (VEGF), and the in vivo regional VEGF levels showed a significant increase after the transplantation. These results suggest that the improvement is not the result of generation of transplanted cell-derived endothelial cells or cardiomyocytes; and raise the possibility that angiogenic cytokines secreted from transplanted cells potentiate angiogenic activity of endogenous cells.
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所属学協会

 4

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 3
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