大学院医学研究院

白澤 浩

シラサワ ヒロシ  (Hiroshi Shirasawa)

基本情報

所属
千葉大学 大学院医学研究院 教授
学位
医学博士(千葉大学)

J-GLOBAL ID
200901055639615933
researchmap会員ID
1000009684

論文

 81
  • XUE MA, TOMOKO OGAWA, ZHENG TIAN, RUIRONG YI, KANG TANG, KENGO SAITO, SARA YATABE, YOSHIFUMI OHNO, RYOSUKE MUROYAMA, EIJI IDO, HISAHIRO MATSUBARA, HIROSHI SHIRASAWA
    Anticancer Research 43(7) 2923-2932 2023年6月23日  
  • YOSHIFUMI OHNO, RUIRONG YI, AKIKO SUGANAMI, YUTAKA TAMURA, AKIO MATSUMOTO, SHOJI MATSUMOTO, KENGO SAITO, HIROSHI SHIRASAWA
    Anticancer Research 41(2) 699-706 2021年2月  
  • Ruirong Yi, Yoshifumi Ohno, Zheng Tian, Shuhan Guo, Weiwei Chen, Xue Ma, Nan Win, Qisen Li, Majid Vahed, Kengo Saito, Shingo Nakamoto, Akiko Suganami, Naohisa Isegawa, Keisuke Yoshida, Shinji Harada, Yutaka Tamura, Atsushi Nishida, Hiroshi Shirasawa
    Oncology Reports 44(6) 2770-2782 2020年10月13日  
  • Eriko Y. Saito, Kengo Saito, Tomoro Hishiki, Ayako Takenouchi, Takeshi Saito, Yoshiharu Sato, Keita Terui, Tadashi Matsunaga, Hiroshi Shirasawa, Hideo Yoshida
    Pediatric Surgery International 36(10) 1173-1180 2020年10月1日  
    Purpose: Oncolytic viral therapy for neuroblastoma (NB) cells with Sindbis virus (SINV) is a promising strategy for treating high-risk NB. Here, we evaluated the possibility of using SINV structural proteins as therapeutic agents for NB since UV-inactivated SINV could induce cytopathogenic effects. Methods: The cytotoxicity of UV-inactivated SINV toward human NB cell lines NB69, NGP, GOTO, NLF, SK-N-SH, SH-SY5Y, CHP134, NB-1, IMR32, and RT-BM-1 were analyzed. Apoptosis was confirmed by TUNEL assays. To determine the components of SINV responsible for the cytotoxicity of UV-inactivated SINV, expression vectors encoding the structural proteins, namely capsid, E2, and E1, were transfected in NB cells. Cytotoxicity was evaluated by MTT assays. Results: UV-inactivated SINV elicited more significant cytotoxicity in NB69, NGP, and RT-BM-1 than in normal human fibroblasts. Results of the transfection experiments showed that all NB cell lines susceptible to UV-inactivated SINV were highly susceptible to the E1 protein, whereas fibroblasts transfected with vectors harboring capsid, E1, or E2 were not. Conclusions: We demonstrated that the cytotoxicity of the UV-inactivated SINV is due to apoptosis induced by the E1 structural protein of SINV, which can be used selectively as a therapeutic agent for NB.
  • Eriko Y Saito, Kengo Saito, Tomoro Hishiki, Ayako Takenouchi, Takeshi Saito, Yoshiharu Sato, Keita Terui, Tadashi Matsunaga, Hiroshi Shirasawa, Hideo Yoshida
    Pediatric surgery international 36(10) 1173-1180 2020年10月  
    PURPOSE: Oncolytic viral therapy for neuroblastoma (NB) cells with Sindbis virus (SINV) is a promising strategy for treating high-risk NB. Here, we evaluated the possibility of using SINV structural proteins as therapeutic agents for NB since UV-inactivated SINV could induce cytopathogenic effects. METHODS: The cytotoxicity of UV-inactivated SINV toward human NB cell lines NB69, NGP, GOTO, NLF, SK-N-SH, SH-SY5Y, CHP134, NB-1, IMR32, and RT-BM-1 were analyzed. Apoptosis was confirmed by TUNEL assays. To determine the components of SINV responsible for the cytotoxicity of UV-inactivated SINV, expression vectors encoding the structural proteins, namely capsid, E2, and E1, were transfected in NB cells. Cytotoxicity was evaluated by MTT assays. RESULTS: UV-inactivated SINV elicited more significant cytotoxicity in NB69, NGP, and RT-BM-1 than in normal human fibroblasts. Results of the transfection experiments showed that all NB cell lines susceptible to UV-inactivated SINV were highly susceptible to the E1 protein, whereas fibroblasts transfected with vectors harboring capsid, E1, or E2 were not. CONCLUSIONS: We demonstrated that the cytotoxicity of the UV-inactivated SINV is due to apoptosis induced by the E1 structural protein of SINV, which can be used selectively as a therapeutic agent for NB.

MISC

 274

共同研究・競争的資金等の研究課題

 37