研究者業績

秋田 新介

Shinsuke Akita

基本情報

所属
千葉大学 医学部附属病院 形成美容外科 講師 (診療准教授)
(兼任)フロンティア医工学センター 講師
学位
博士(医学)(2013年3月 千葉大学)

J-GLOBAL ID
201901006965868309
researchmap会員ID
B000367861

Surgeon-scientistとして、形成外科学を、形態の異常や組織の変性にどこまで抗うことができるかを探求・挑戦する領域ととらえています。臨床医学の疑問を基礎研究に立ち戻って検証し、基礎研究で得られた知見を臨床医学に応用することを基本姿勢としています。現在は、特にリンパ管系を中心とした皮膚と皮下組織の変性、線維化、老化の理解と、その治療・再建にフォーカスしています。生物学的な手法と医工学的な手法を駆使し、病態の本質的な理解と、革新的な治療方法の開発や安全な治療マネージメント方法の確立を目指して研究に取り組んでいます。


学歴

 2

論文

 193
  • Mizuna Aisawa, Yoshitaka Kubota, Kazuhiko Azuma, Syota Ohki, Kentaro Ishii, Yoshihisa Yamaji, Shinsuke Akita, Yuzuru Ikehara, Nobuyuki Mitsukawa
    PLASTIC AND RECONSTRUCTIVE SURGERY-GLOBAL OPEN 13(3) 2025年3月  
    Absorbable bone fixation materials are commonly used in facial bone fracture surgery. However, reports on the microstructure of infected implants are rare. This study presents a case involving a 74-year-old male patient who developed an alpha-Streptococcus infection 2.5 months after zygomatic bone fracture surgery and the ultrastructure of an infected absorbable plate observed under a scanning electron microscope (SEM). The patient sustained a right zygomatic bone fracture due to a fall and underwent open reduction and internal fixation with SonicWeld on the seventh day after the injury. The postoperative course was uneventful, but 2.5 months after the surgery, the patient presented with redness, swelling, and pain in the right lower eyelid, along with purulent discharge, leading to the removal of the bone fixation material. The SEM observation of the extracted implant revealed a biphasic pattern, with areas showing signs of infection and areas without such effects. The infected regions showed wide cracks, bacterial clusters, and biofilm formation, whereas noninfected areas exhibited narrow cracks and distinct geometric striations. These findings suggest a link between crack formation and bacterial proliferation, emphasizing the importance of proper handling and implantation techniques. Although the infection was controlled and bone healing achieved after the removal of infected implants, the patient experienced lower eyelid retraction due to adhesion between the skin and underlying bone, leading to persistent cosmetic issues. This case report presents a rare visualization of the ultrastructural characteristics of infected absorbable implants, offering insights into the potential role of SEM in understanding these changes and processes.
  • Arbariyanto Mahmud Wicaksono, Daisuke Kawashima, Ryoma Ogawa, Shinsuke Akita, Masahiro Takei
    Biomedical physics & engineering express 11(2) 2025年1月31日  
    Albumin andγ-globulin concentrations in subcutaneous adipose tissues (SAT) have been quantified by multivariate regression based on admittance relaxation time distribution (mrARTD) under the fluctuated background of sodium electrolyte concentration. ThemrARTD formulatesP=Ac+Ξ(P: peak matrix of distribution function magnitudeγˆand relaxation timesτˆ,c: concentration matrix of albumincAlb,γ-globulinGloc, and sodium electrolyteNac,A: coefficient matrix of a multivariate regression model, andΞ: error matrix). ThemrARTD is implemented by two processes which are: (1) the training process ofAthrough the maximum likelihood estimation ofPand (2) the quantification process ofcAlb,Gloc, andNacthrough the model prediction. In the training process, a positive correlation is present betweencAlb,Gloc, andNactoγˆ1atτˆ1= 0.1 as well asγˆ2atτˆ2= 1.40 μs as under a fixed concentration of proteins solution into a porcine SAT (cAlb= 0.800-2.400 g/dL,Gloc= 0.400-1.200 g dl-1andNac= 0.700-0.750 g dl-1). ThemrARTD method quantifiescAlb,Gloc, andNacin SAT with an absolute error of 33.79%, 44.60%, and 2.18%, respectively.
  • Tatsuya Ishigaki, Shinsuke Akita, Akikazu Udagawa, Hiroyuki Suzuki, Nobuyuki Mitsukawa
    Journal of orthopaedic science : official journal of the Japanese Orthopaedic Association 29(6) 1406-1410 2024年11月  
    PURPOSE: Keloid formation in toes area is rare. However, occurrence of this phenomenon in toes after the surgery of syndactyly repair has been reported. Risk factors of keloid formation in toes after syndactyly reconstructions are currently unknown. This study aimed to investigate the risk factors of keloid formation after the surgery of syndactyly repair of the toes. METHODS: We retrospectively reviewed our case series including patients who were treated surgically at our institution. We hypothesized some key factors of keloid formation and analyzed each of them statistically. RESULTS: A total of 105 patients were treated surgically at our hospital, and 9 patients were involved keloid formations after operations. Among our hypothesized key factors, the results of multivariate logistic regression analysis revealed the number of affected web spaces (OR 0.031; 95%CI 0.001-0.684; p = 0.028) was significantly different. Digital enlargement was not a significant factor (OR 17.731; 95%CI 0.686-458.174; p = 0.091). CONCLUSION: Involving multiple web space was associated with keloid formation after syndactyly release, on the other hand, toe enlargement did not show a significant difference. However, the digital enlargement showed high Odds ratio, we could not deny its high relativity for keloid formation. Further investigations are needed to clarify the key risk factors of keloid formation after the surgery of syndactyly repair of the toes.
  • Kentaro Kosaka, Naoya Takayama, Sudip Kumar Paul, Maria Alejandra Kanashiro, Motohiko Oshima, Masaki Fukuyo, Bahityar Rahmutulla, Ikuko Tajiri, Michiaki Mukai, Yoshitaka Kubota, Shinsuke Akita, Nobutaka Furuyama, Atsushi Kaneda, Atsushi Iwama, Koji Eto, Nobuyuki Mitsukawa
    Stem cell research & therapy 15(1) 364-364 2024年10月14日  
    BACKGROUND: Platelet-rich plasma (PRP), which is prepared by concentrating platelets in autologous blood, shows efficacy in chronic skin wounds via multiple growth factors. However, it exhibits heterogeneity across patients, leading to unstable therapeutic efficacy. Human induced pluripotent stem cell (iPSC)-derived megakaryocytes and platelets (iMPs) are capable of providing a stable supply, holding promise as materials for novel platelet concentrate-based therapies. In this context, we evaluated the effect of iMPs on wound healing and validated lyophilization for clinical applications. METHODS: The growth factors released by activated iMPs were measured. The effect of the administration of iMPs on human fibroblasts and human umbilical vein endothelial cells (HUVECs) was investigated in vitro. iMPs were applied to dorsal skin defects of diabetic mice to assess the wound closure rate and quantify collagen deposition and angiogenesis. Following the storage of freeze-dried iMPs (FD-iMPs) for three months, the stability of growth factors and their efficacy in animal models were determined. RESULT: Multiple growth factors that promote wound healing were detected in activated iMPs. iMPs specifically released FGF2 and exhibited a superior enhancement of HUVEC proliferation compared to PRP. Moreover, an RNA-seq analysis revealed that iMPs induce polarization to stalk cells and enhance ANGPTL4 gene expression in HUVECs. Animal studies demonstrated that iMPs promoted wound closure and angiogenesis in chronic wounds caused by diabetes. We also confirmed the long-term stability of growth factors in FD-iMPs and their comparable effects to those of original iMPs in the animal model. CONCLUSION: Our study demonstrates that iMPs promote angiogenesis and wound healing through the activation of vascular endothelial cells. iMPs exhibited more effectiveness than PRP, an effect attributed to the exclusive presence of specific factors including FGF2. Lyophilization enabled the long-term maintenance of the composition of the growth factors and efficacy of the iMPs, therefore contributing to stable supply for clinical application. These findings suggest that iMPs provide a novel treatment for chronic wounds.
  • Saito Sakaguchi, Moe Tsutsumi, Shinsuke Akita, Masashi Konyo, Kentaro Kajiya
    The Journal of investigative dermatology 144(10) 2332-2335 2024年10月  

MISC

 307

講演・口頭発表等

 139

共同研究・競争的資金等の研究課題

 20

社会貢献活動

 12