大学院医学研究院

陳 思婧

チェン スジン  (Si Jing Chen)

基本情報

所属
千葉大学 大学院医学研究院, 先端研究部門, イノベーション再生医学 特任助教
学位
博士(農学)(2016年3月 九州大学)

研究者番号
90899577
ORCID ID
 https://orcid.org/0000-0002-7857-1140
J-GLOBAL ID
202301016629653025
researchmap会員ID
R000059875

論文

 8
  • Si Jing Chen, Kazuya Hashimoto, Kosuke Fujio, Karin Hayashi, Sudip Kumar Paul, Akinori Yuzuriha, Wei-Yin Qiu, Emiri Nakamura, Maria Alejandra Kanashiro, Mio Kabata, Sou Nakamura, Naoshi Sugimoto, Atsushi Kaneda, Takuya Yamamoto, Hirohide Saito, Naoya Takayama, Koji Eto
    Nature Communications 15(1) 2024年3月22日  査読有り筆頭著者
    Abstract We recently achieved the first-in-human transfusion of induced pluripotent stem cell-derived platelets (iPSC-PLTs) as an alternative to standard transfusions, which are dependent on donors and therefore variable in supply. However, heterogeneity characterized by thrombopoiesis-biased or immune-biased megakaryocytes (MKs) continues to pose a bottleneck against the standardization of iPSC-PLT manufacturing. To address this problem, here we employ microRNA (miRNA) switch biotechnology to distinguish subpopulations of imMKCLs, the MK cell lines producing iPSC-PLTs. Upon miRNA switch-based screening, we find imMKCLs with lower let-7 activity exhibit an immune-skewed transcriptional signature. Notably, the low activity of let-7a-5p results in the upregulation of RAS like proto-oncogene B (RALB) expression, which is crucial for the lineage determination of immune-biased imMKCL subpopulations and leads to the activation of interferon-dependent signaling. The dysregulation of immune properties/subpopulations, along with the secretion of inflammatory cytokines, contributes to a decline in the quality of the whole imMKCL population.
  • Si Jing Chen, Naoshi Sugimoto, Koji Eto
    International Journal of Hematology 117(3) 349-355 2023年3月  査読有り筆頭著者責任著者
  • Hideya Seo, Si Jing Chen, Kazuya Hashimoto, Hiroshi Endo, Yohei Nishi, Akira Ohta, Takuya Yamamoto, Akitsu Hotta, Akira Sawaguchi, Hideki Hayashi, Noritaka Koseki, George J. Murphy, Kazuhiko Fukuda, NAOSHI SUGIMOTO, Koji Eto
    Blood Advances 2(17) 2262-2272 2018年9月11日  査読有り筆頭著者
  • Si Jing Chen, Chiwa Aikawa, Risa Yoshida, Tomoaki Kawaguchi, Toshiro Matsui
    Journal of the Science of Food and Agriculture 97(12) 3923-3928 2017年9月  査読有り筆頭著者
  • Inoue H, Nishio H, Takada H, Sakai Y, Nanishi E, Ochiai M, Onimaru M, Chen SJ, Matsui T, Hara T
    Journal of Immunology 2016年2月  査読有り

共同研究・競争的資金等の研究課題

 3