荒井 孝義

Abstract In a tris(pentafluorophenyl)borane [B(C₆F₅)₃]‐catalyzed reaction of alkenes with cyanogen halides (XCN), BrCN proceeds in a cyanation/bromination manner, in which a cationic CN character is realized. The reaction using ICN switched the mode to iodination/cyanation for enamide substrates and electron‐enriched styrenes. The origin of the iodination/cyanation is the B(C₆F₅)₃‐enhanced σ‐hole generated on ICN.

G-quadruplexes (G4s) regulate various biological processes in cells. However, cellular imaging of dynamically forming G4s in biomolecular condensates using small molecules has been poorly investigated. Herein, we present a fluorescent light-up probe with the ability to selectively stabilize G4s and enhance fluorescence upon G4 binding. The foci of the probe were mainly observed in the nucleoli. These were co-localized with anti-fibrillarin antibodies and anti-G4 antibodies (BG4). Moreover, we tested detection of G4 in stress granules using the developed probe. Stress granules were induced through treatment with not only thapsigargin, but also known G4 ligands (pyridostatin, RHPS4, and BRACO-19). In the stress granules, co-localization between the probe, BG4, and stress granule markers (TIA1 and G3BP1) was detected. We present a practical light-up probe for G4s in stress granules, providing potential targets for G4 ligands.