Normal sperm flagellar shape and movement are essential for fertilization. The integral protein outer dense fiber 4 (ODF4) localizes to ODFs, but its function remains unclear. Adenylate kinase (AK) is a phosphotransferase that catalyzes ...
Lysyl hydroxylase 2 (LH2) is an enzyme that catalyzes the hydroxylation of lysine (Lys) residues in fibrillar collagen telopeptides, a critical post-translational modification for the stability of intermolecular cross-links. Though abnormal LH2 ac...
Deleted in lung and esophageal cancer 1 (DLEC1) is a tumour suppressor gene that is downregulated in various cancers in humans; however, the physiological and molecular functions of DLEC1 are still unclear. This study investigated the critical rol...
Biology of Reproduction 102(3) 750-757 2020年3月 [査読有り]
<title>Abstract</title>
The acrosome reaction is a multi-step event essential for physiological fertilization. During the acrosome reaction, gamete fusion-related factor IZUMO1 translocates from the anterior acrosome to the equatorial segment and...
Outer dense fibre 2 (Odf2 or ODF2) is a cytoskeletal protein required for flagella (tail)-beating and stability to transport sperm cells from testes to the eggs. There are infertile males, including human patients, who have a high percentage of de...
Y Iwamoto   T Kaneko   J Ichinose   T Mori   Y Shibata   K Toshimori   H Iida   
BIOLOGY OF REPRODUCTION 72(2) 284-292 2005年2月
We used differential display in combination with cDNA cloning to isolate a novel rat gene, designated as Spetex2, that has an open reading frame of 582 nucleotides, encoding a protein of 194 amino acids. Spetex2 mRNA was highly expressed in testis...
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 324(1) 147-153 2004年11月
The induction of null mutations by means of homologous recombination is a powerful technique for clarifying the biological activities of target genes. However, the problems of the genetic background and flanking genes should be borne in mind. Here...
Archives of Histology and Cytology 67(4) 307-314 2004年11月
Cnot7 is a co-factor of transcription regulation, expressed in a variety of tissues including the lung, liver, thyroid gland, and testis. Our previous study (Nakamura et al., 2004) showed that deletion of the Cnot7 gene in mice caused almost no ab...
Chizuru Ito   Fumie Suzuki-Toyota   Mamiko Maekawa   Yoshiro Toyama   Ryoji Yao   Tetsuo Noda   Kiyotaka Toshimori   
Archives of Histology and Cytology 67(4) 349-360 2004年11月
Deletion of the GOPC gene encoding mouse GOPC (Golgi-associated PDZ- and coiled-coil motif-containing protein) causes infertile round-headed spermatozoa, which have acrosome-less round heads and deformed tails (Yao et al, 2002). This study investi...
Fumie Suzuki-Toyota   Chizuru Ito   Yoshiro Toyama   Mamiko Maekawa   Ryoji Yao   Tetsuo Noda   Kiyotaka Toshimori   
Archives of Histology and Cytology 67(4) 361-371 2004年11月
Male mice deleting the gene encoding GOPC (Golgi-associated PDZ- and coiled-coil motif-containing protein) are infertile, showing globozoospermia with a coiled tail (Yao et al., 2002). We confirmed how and where tail anomalies were produced in spe...