Tremendous efforts have been made to develop practical and efficient microfluidic cell and particle sorting systems; however, there are technological limitations in terms of system complexity and low operability. Here, we propose a sheath flow gen...
Although droplet microfluidics has been studied for the past two decades, its applications are still limited due to the low productivity of microdroplets resulting from the low integration of planar microchannel structures. In this study, a microf...
Journal of Bioscience and Bioengineering 135(5) 417-422 2023年5月
Technologies for efficiently expanding Chinese hamster ovary (CHO) cells, the primary host cells for antibody production, are of growing industrial importance. Various processes for the use of microcarriers in CHO suspension cultures have been dev...
Mai Takagi   Masumi Yamada   Rie Utoh   Minoru Seki   
Lab on a Chip 23(9) 2257-2267 2023年5月
Spheroid formation assisted by microengineered chambers is a versatile approach for morphology-controlled three-dimensional (3D) cell cultivation with physiological relevance to human tissues. However, the limitation in diffusion-based oxygen/nutr...
Numerous attempts have been made to develop efficient systems to purify trace amounts of circulating tumor cells (CTCs) from blood samples. However, current technologies are limited by complexities in device fabrication, system design, and process...
Takeru Sato   Yurika Sakurai   Masumi Yamada   Minoru Seki   
MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences 753-754 2020年
Here we present a medium exchange system for cell culture applications using microfluidic devices partially made of a micropored substrate as fluid drainage. The microdevices were fabricated by bonding a microchannel-embedding PDMS plate with a pa...
Yurika Sakurai   Takeru Sato   Masumi Yamada   Minoru Seki   
MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences 643-644 2020年
Here we propose a new scheme for particle sorting using cross-flow microfluidic devices incorporating a porous PDMS substrate. The porous region, prepared by dissolving sacrificial particles encapsulated in the PDMS prepolymer, allows small partic...
Mai Takagi   Masumi Yamada   Minoru Seki   
MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences 849-850 2020年
Here we present a unique perfusion cell culture system capable of forming uniformly-sized multicellular aggregates utilizing microcavities embedded in a spongelike PDMS matrix. The combination of a particle leaching method and a replica molding te...
MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences 731-732 2020年
Here we propose an efficient approach to processing mammalian cells using microfluidic devices integrated with spongious PDMS matrices having continuous micropores. The micropores on the sponge surface created by dissolving sacrificial micropartic...