Tremendous efforts have been made to develop practical and efficient microfluidic cell and particle sorting systems; however, there are technological limitations in terms of system complexity and low operability. Here, we propose a sheath flow gen...
Although droplet microfluidics has been studied for the past two decades, its applications are still limited due to the low productivity of microdroplets resulting from the low integration of planar microchannel structures. In this study, a microf...
Journal of Bioscience and Bioengineering 135(5) 417-422 2023年5月
Technologies for efficiently expanding Chinese hamster ovary (CHO) cells, the primary host cells for antibody production, are of growing industrial importance. Various processes for the use of microcarriers in CHO suspension cultures have been dev...
Mai Takagi   Masumi Yamada   Rie Utoh   Minoru Seki   
Lab on a Chip 23(9) 2257-2267 2023年5月
Spheroid formation assisted by microengineered chambers is a versatile approach for morphology-controlled three-dimensional (3D) cell cultivation with physiological relevance to human tissues. However, the limitation in diffusion-based oxygen/nutr...
Numerous attempts have been made to develop efficient systems to purify trace amounts of circulating tumor cells (CTCs) from blood samples. However, current technologies are limited by complexities in device fabrication, system design, and process...
23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019 608-609 2019年
Here we proposed a new high-throughput, continuous microfluidic device for particle/cell separation based on pinched flow fractionation. In the newly-proposed system, a wide and shallow microchannel is fabricated and particles are separated in the...