Journal of Bioscience and Bioengineering 135(5) 417-422 2023年5月
Technologies for efficiently expanding Chinese hamster ovary (CHO) cells, the primary host cells for antibody production, are of growing industrial importance. Various processes for the use of microcarriers in CHO suspension cultures have been dev...
Mai Takagi   Masumi Yamada   Rie Utoh   Minoru Seki   
Lab on a Chip 23(9) 2257-2267 2023年5月
Spheroid formation assisted by microengineered chambers is a versatile approach for morphology-controlled three-dimensional (3D) cell cultivation with physiological relevance to human tissues. However, the limitation in diffusion-based oxygen/nutr...
Numerous attempts have been made to develop efficient systems to purify trace amounts of circulating tumor cells (CTCs) from blood samples. However, current technologies are limited by complexities in device fabrication, system design, and process...
Journal of Bioscience and Bioengineering 133(3) 265-272 2022年3月
Numerous attempts have been made to organize isolated primary hepatocytes into functional three-dimensional (3D) constructs, but technologies to introduce extracellular matrix (ECM) components into such assemblies have not been fully developed. He...
23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019 675-676 2019年
Here we present lateral flow immunoassay platforms consisted of polymeric sheets with micro/nanoimprinted multiscale architectures. Microcone arrays with nanometer-scale surface roughness were prepared by thermal nano-imprinting. The dramatically ...
23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019 282-283 2019年
Here we present a unique process to fabricate type-I collagen hydrogels integrated in microfluidic devices, with the help of gelation agent-incorporating composite PDMS substrates. We fabricated microfluidic devices made of phosphate particle-embe...
23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019 592-593 2019年
Here we present a particle separation system utilizing microengineered PDMS plates with micrometer-sized continuous pores as the sieving matrix. The continuous micropores were formed using sacrificial microparticles embedded in and dissolved from ...
23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019 326-327 2019年
Although various types of hydrogel-based 3D cell culture systems have been developed, creation of micrometer-sized capillaries as the conduits in bulk-scale hydrogels still remains a challenge. Here we present a highly efficient strategy to form c...
MHS 2018 - 2018 29th International Symposium on Micro-NanoMechatronics and Human Science 2018年12月
Although various types of hydrogel-based 3D cell culture platforms have been developed, it is difficult to prepare porous hydrogels encapsulating intact cells and having capillary networks with a one-step procedure. Here we propose a facile strate...