Journal of Bioscience and Bioengineering 135(5) 417-422 2023年5月
Technologies for efficiently expanding Chinese hamster ovary (CHO) cells, the primary host cells for antibody production, are of growing industrial importance. Various processes for the use of microcarriers in CHO suspension cultures have been dev...
Spheroid formation assisted by microengineered chambers is a versatile approach for morphology-controlled three-dimensional (3D) cell cultivation with physiological relevance to human tissues. However, the limitation in diffusion-based oxygen/nutr...
Numerous attempts have been made to develop efficient systems to purify trace amounts of circulating tumor cells (CTCs) from blood samples. However, current technologies are limited by complexities in device fabrication, system design, and process...
Journal of Bioscience and Bioengineering 133(3) 265-272 2022年3月
Numerous attempts have been made to organize isolated primary hepatocytes into functional three-dimensional (3D) constructs, but technologies to introduce extracellular matrix (ECM) components into such assemblies have not been fully developed. He...
MHS 2018 - 2018 29th International Symposium on Micro-NanoMechatronics and Human Science 2018年12月
Here we present a new process to prepare cell co-culture platforms incorporating thin extracellular matrix (ECM) membranes. We utilized PDMS microstencil plates to generate thin ECM membranes, which were physically stabilized in the micro through ...
MHS 2017 - 28th 2017 International Symposium on Micro-NanoMechatronics and Human Science 2018-January 1-4 2018年2月
In this study, we propose a new cell culture system using microengineered hierarchical hydrogel sheets, which enables guidance and evaluation of cancer cell invasion in 3D environments. Three-layered hydrogel sheets, which encapsulated cancer cell...
22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 3 1428-1430 2018年
Although many types of hydrogel-based cell culture platforms have been proposed, techniques for preparing microvasculature-embedded hydrogels haven’t been fully developed. Here we propose a facile and versatile strategy to fabricate hydrogel spong...
22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 3 1442-1444 2018年
Here we present a microfluidic system to produce linear cell assemblies, which were simultaneously wrapped with thin collagen membranes, using multilayered microfluidic devices. A cell suspension with a polymeric thickener (for core) and an aqueou...
大学院国際学術研究院