Journal of Bioscience and Bioengineering 133(3) 265-272 2022年3月
Numerous attempts have been made to organize isolated primary hepatocytes into functional three-dimensional (3D) constructs, but technologies to introduce extracellular matrix (ECM) components into such assemblies have not been fully developed. He...
Although many types of technologies for hydrogel-based cell cultivation have recently been developed, strategies to integrate cell-adhesive micrometer-sized supports with bulk-scale hydrogel platforms have not been fully established. Here, we pres...
Visualization and quantification of intracellular molecules of mammalian cells are crucial steps in clinical diagnosis, drug development, and basic biological research. However, conventional methods rely mostly on labor-intensive, centrifugation-b...
Lateral flow immunoassay devices have revolutionized the style of on-site disease detection and point-of-care testing in the past few decades. The surface nanotopography of a solid substrate is a dominant parameter in the efficiency of antibody im...
21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017 177-178 2020年
A new process is presented to fabricate basement membrane-mimetic cell co-culture platforms using microfabricated PDMS stencil plates. An aqueous solution of extracellular matrices (ECM) was introduced into the through-holes of the microstencil pl...
21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017 1145-1146 2020年
Here we present a new approach to rapid formation of collagen gel tubes using microfluidic devices made of phosphate particle-embedding PDMS (PP-PDMS). An acidic solution of collagen, introduced into the PP-PDMS channel, was rapidly transformed in...
MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences 1155-1156 2020年
Here we present an efficient microfluidic system for selectively capturing circulating tumor cells (CTCs) from blood samples, employing parallelized structures of cell trappers. The thin configuration of the trapper (depth of several micrometers) ...