We herein propose a new hydrodynamic mechanism of particle separation using dual-depth, lattice-patterned asymmetric microchannel networks. This mechanism utilizes three-dimensional (3D) laminar flow profiles formed at intersections of lattice cha...
Even though a number of microfluidic systems for particle/cell sorting have been proposed, facile and versatile platforms that provide sufficient sorting throughput and good operability are still under development. Here we present a simple but eff...
With the recent progress in three-dimensional (3D) cell culture techniques for regenerative medicine and drug development, hydrogel-based tissue engineering approaches that can precisely organize cells into functional formats have attracted increa...
Sensors and Actuators, B: Chemical 287 486-495 2019年5月
Thin membrane-based cell culture platforms have recently gained much attention for facilitating separated but adjacent cocultures of heterotypic cells in in vivo tissue-mimetic microenvironments. Here we propose a new approach to preparing signifi...
Although various types of on-site immunoassay platforms have been developed, facile and reliable sample-to-answer immunoassay systems are still under development. In this study, we proposed a lateral-flow immunoassay system utilizing a polymer she...
21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017 177-178 2020年
A new process is presented to fabricate basement membrane-mimetic cell co-culture platforms using microfabricated PDMS stencil plates. An aqueous solution of extracellular matrices (ECM) was introduced into the through-holes of the microstencil pl...
21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017 1145-1146 2020年
Here we present a new approach to rapid formation of collagen gel tubes using microfluidic devices made of phosphate particle-embedding PDMS (PP-PDMS). An acidic solution of collagen, introduced into the PP-PDMS channel, was rapidly transformed in...
MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences 1155-1156 2020年
Here we present an efficient microfluidic system for selectively capturing circulating tumor cells (CTCs) from blood samples, employing parallelized structures of cell trappers. The thin configuration of the trapper (depth of several micrometers) ...