Journal of Bioscience and Bioengineering 135(5) 417-422 2023年5月 [査読有り]
Technologies for efficiently expanding Chinese hamster ovary (CHO) cells, the primary host cells for antibody production, are of growing industrial importance. Various processes for the use of microcarriers in CHO suspension cultures have been dev...
Mai Takagi   Masumi Yamada   Rie Utoh   Minoru Seki   
LAB ON A CHIP 23(9) 2257-2267 2023年5月 [査読有り]
Spheroid formation assisted by microengineered chambers is a versatile approach for morphology-controlled three-dimensional (3D) cell cultivation with physiological relevance to human tissues. However, the limitation in diffusion-based oxygen/nutr...
Journal of Bioscience and Bioengineering 133(3) 265-272 2022年3月 [査読有り]
Numerous attempts have been made to organize isolated primary hepatocytes into functional three-dimensional (3D) constructs, but technologies to introduce extracellular matrix (ECM) components into such assemblies have not been fully developed. He...
Numerous attempts have been made to develop efficient systems to purify trace amounts of circulating tumor cells (CTCs) from blood samples. However, current technologies are limited by complexities in device fabrication, system design, and process...
Selection of specific cells based on multiple factors is an essential process for clinical diagnosis/treatment and biological research. In this study, we propose a simple microfluidic cell separator based on two factors (size and surface marker), ...
Construction of large tissues from cell aggregates is one of the promising methods for tissue engineering applications. Compared to spherical cell aggregates, non-spherical cell aggregates are more suitable as building blocks to be assembled into ...