The glomerular map in the olfactory bulb (OB) is the basis for odor recognition. Once established during development, the glomerular map is stably maintained throughout the life of an animal despite the continuous turnover of olfactory sensory neu...
Masakazu Hashimoto Tatsuya Sato Yuko Muroyama Lisa Fujimura Masahiko Hatano Tetsuichiro Saito
Development Growth & Differentiation 57(7) 529-538 2015年9月 [査読有り]
We generated knockout (KO) mice of Nepro, which has been shown to be necessary to maintain neural progenitor cells downstream of Notch in the mouse developing neocortex by using knockdown experiments, to explore its function in embryogenesis. Nepr...
Consistent and efficient gene transfer into postmitotic neurons is desirable for the study of neurons. Recent refinement of cell culturing techniques has enabled us to prepare various types of neurons in dissociated cultures. Among them, hippocamp...
In utero electroporation, which was developed by combining electroporation with in utero surgery, has greatly facilitated functional analyses of genes through gain-of-function and loss-of-function approaches. DNA and RNA are efficiently transfecte...
Quick and efficient transfection of nucleic acids by in utero electroporation has revolutionized gene analyses in the mammalian brain. Microinjection of nucleic acids into a targeted site, however, is not easy, because young embryos are not clearl...
大学院国際学術研究院