The transcription factor Sox2 is expressed at high levels in neural stem and progenitor cells. Here, we inactivated Sox2 specifically in the developing brain by using Cre-loxP system. Although mutant animals did not survive after birth, analysis o...
S Miyagi M Nishimoto T Saito M Ninomiya K Sawamoto H Okano M Muramatsu H Oguro A Iwama A Okuda
JOURNAL OF BIOLOGICAL CHEMISTRY 281(19) 13374-13381 2006年5月 [査読有り]
Sox2 is expressed at high levels in neuroepithelial stem cells and persists in neural stem/ progenitor cells throughout adulthood. We showed previously that the Sox2 regulatory region 2 ( SRR2) drives strong expression in these cells. Here we gene...
D Kawauchi H Taniguchi H Watanabe T Saito F Murakami
DEVELOPMENT 133(6) 1113-1123 2006年3月 [査読有り]
Nuclei are aggregates of neurons distributed in the central nervous system and are fundamental functional units that share anatomical and physiological features. Despite their importance, the cellular basis that leads to nucleogenesis is only poor...
This protocol describes a basic method for in vivo electroporation in the nervous system of embryonic mice. Delivery of electric pulses following microinjection of DNA into the brain ventricle or the spinal cord central canal enables efficient tra...
Proneural basic helix-loop-helix (bHLH) proteins are key regulators of neurogenesis. However, downstream target genes of the bHLH proteins remain poorly defined. Mbh1 confers commissural neuron identity in the spinal cord. Enhancer analysis using ...
大学院国際学術研究院