研究者業績

矢口 貴志

ヤグチ タカシ  (Takashi Yaguchi)

基本情報

所属
千葉大学 真菌医学研究センター 准教授
学位
博士(工学)(早稲田大学)

J-GLOBAL ID
200901010374246864
researchmap会員ID
5000067659

論文

 264
  • Tetsuhiro Matsuzawa, Reiko Tanaka, Yoshikazu Horie, Tohru Gonoi, Takashi Yaguchi
    Japanese Journal of Medical Mycology 51(2) 109-116 2010年  
    Aspergillosis is an important mycosis caused primarily by Aspergillus fumigatus and its relatives. The genus Emericella is a teleomorph related to the Aspergillus section Nidulantes. The typical anamorphic stage species in this genus is Aspergillus nidulans, which is sometimes a significant agent in chronic granulomatous disease (CGD) patients. The mortality rate of osteomyelitis in CGD patients due to A. nidulans (E. nidulans) is very high compared to that due to A. fumigatus. Moreover, two Emericella species (E. nidulans and E. quadrilineata) from clinical specimens exhibit different sensitivities against several antifungal drugs. In aspergillosis, correct species identification is important for antifungal therapy. We attempted to develop rapid and specific molecular discrimination by polymerase chain reaction (PCR) and loop-mediated isothermal amplification (LAMP) methods in the principal pathogenic Emericella species, and succeeded in establishing species-specific primers corresponding to the hydrophobin gene. These primers discriminate E. nidulans and E. quadrilineata rapidly and specifically. These methods and primers make it possible to diagnose etiological agents in aspergillosis quickly and easily.
  • Juhaer Mijiti, Xiong Ming Pu, Alshat Erfan, Takashi Yaguchi, Hiroji Chibana, Reiko Tanaka
    Nihon Ishinkin Gakkai zasshi = Japanese journal of medical mycology 51(3) 165-8 2010年  
    Antifungal susceptibility tests were performed for 38 Candida albicans strains isolated from oral cavities of 43 Uighurian AIDS patients. Results showed that six isolates were resistant to fluconazole; one showed low susceptibility. We attempted to examine these strains molecular-epidemiologically, but 25S rDNA genotyping was insufficient for their discrimination. To estimate whether the origins of resistant strains were identical, we developed a new combination method of C. albicans tandem repeating units (ALTS)/RFLP and micro-temperature gradient gel electrophoresis (micro-TGGE). This new method was able to distinguish all seven strains. A suspected nosocomial infection was ruled out.
  • Hideo Takahashi, Keiichi Ueda, Eiko Nakagawa Itano, Makio Yanagisawa, Yoshiteru Murata, Michiko Murata, Takashi Yaguchi, Masaru Murakami, Katsuhiko Kamei, Tomo Inomata, Hirokazu Miyahara, Ayako Sano, Senzo Uchida
    Veterinary Medicine International 2010 2010年  
    Genotypes of Candida spp. isolated from exhalation of 20 dolphins, 11 water samples from captive pools, and 24 oral cavities of staff members in an aquarium using a combination of multiple drug resistance 1 gene (MDR1) and the internal transcribed spacer (ITS) 1 5.8s-ITS 2 regions of ribosomal RNA gene (ITS rDNA) sequences were studied. The holding ratios of the dolphins, captive pools, and staff members were 70, 90, and 29, respectively. Isolated pathogenic yeast species common to the dolphins and environments were Candida albicans and C. tropicalis. Identical genotypes in both Candida spp. based on the combination of MDR1 and ITS rDNA were found in some dolphins, between a dolphin and a staff, among dolphins and environments, and among environments. The results indicated the diffusion and exchange of pathogenic yeasts at the aquarium among dolphins and environments. The isolates at the aquarium showed higher rates of resistance to azole antifungals compared to reference isolates. Copyright © 2010 Hideo Takahashi et al.
  • 菊池 和代, 伊藤 純子, 田口 英昭, 渡辺 哲, 矢口 貴志, 亀井 克彦
    日本臨床微生物学雑誌 19(4) 208-208 2009年12月  
  • Pham Minh Duc, Kishio Hatai, Osamu Kurata, Kozue Tensha, Uchida Yoshitaka, Takashi Yaguchi, Shun-Ichi Udagawa
    MYCOPATHOLOGIA 167(5) 229-247 2009年5月  
    Two fungal pathogens of the mantis shrimp (Oratosquilla oratoria) in Yamaguchi and Aichi Prefectures, Japan are described as the new species Plectosporium oratosquillae and Acremonium sp. (a member of the Emericellopsis marine clade). Both fungi infect the gills of the mantis shrimp, which become brown or black due to melanization. The former species is characterized by its slow growth on artificial seawater yeast extract peptone glucose (PYGS) agar, pale yellow to pale orange or grayish yellow colonies, short cylindrical solitary phialides with a wavy tip, and one-celled ellipsoidal conidia. Although lacking the two-celled conidia demonstrated by the type species Plectosporium tabacinum, the taxonomic placement of the new species was confirmed by DNA sequence analysis of the internal transcribed spacer (ITS) region of ribosomal DNA (ITS1, 5.8S rDNA and ITS2). Acremonium sp., the other causal pathogen, differs from P. oratosquillae by its fast growth on PYGS agar, pale orange to salmon-colored colonies, long, slender conidiophores consisting of solitary phialides with tips lacking an undulate outline, and typically cylindrical conidia. Analysis of ITS and beta-tubulin gene sequences placed this fungus within the phylogenetically distinct Emericellopsis (anam. Acremonium) marine clade. Various physiological characteristics of both pathogens were also investigated. This is the first report of a fungal infection found on the mantis shrimp in Japan.
  • Daigo Wakana, Tomoo Hosoe, Hiroshi Wachi, Takeshi Itabashi, Kazutaka Fukushima, Takeshi Yaguchi, Ken-ichi Kawai
    JOURNAL OF ANTIBIOTICS 62(4) 217-219 2009年4月  
  • Takeshi Itabashi, Tomoo Hosoe, Daigo Wakana, Kazutaka Fukushima, Kayoko Takizawa, Takashi Yaguchi, Kaoru Okada, Galba Maria de Campos Takaki, Ken-ichi Kawai
    JOURNAL OF NATURAL MEDICINES 63(1) 96-99 2009年1月  
    In a screen searching for new bioactive agents, a new indoloditerpene, penijanthine A (1), was isolated from Penicillium janthinellum IFM 55557. The structure of 1 was established on the basis of spectroscopic and chemical investigation, as well as detailed comparison with the spectroscopic and physico-chemical data of paxilline (2), which was isolated along with 1.
  • Shou Nakadate, Koohei Nozawa, Hiroyasu Sato, Hitoshi Horie, Yuichi Fujii, Masahiro Nagai, Tomoo Hosoe, Ken-ichi Kawai, Takashi Yaguchi
    JOURNAL OF NATURAL PRODUCTS 71(9) 1640-1642 2008年9月  
    In the course of searching for new antifungal agents, a new cyclic depsipeptide, eujavanicin A (1), wits isolated from Eupenicillium javanicum as an antifungal agent against the human pathogenic filamentous fungus Aspergillus fumigatus. The structure of 1 was established by spectroscopic and chemical investigations. The absolute stereochemistry was elucidated by Marfey's method and by chiral HPLC analysis.
  • Takashi Yaguchi, Masako Takashima, Hiroko Kawasaki, Koichi Makimura
    Japanese Journal of Medical Mycology 49(3) 221-228 2008年8月20日  
  • 亀井 克彦, 矢口 貴志
    Medical Technology 36(7) 695-700 2008年7月  
  • Takashi Yaguchi, Reiko Tanaka
    Nippon rinsho. Japanese journal of clinical medicine 66 2261-2267 2008年1月1日  
    The clinical isolates are traditionally classified based on morphology, but in recent years a polyphasic taxonomy that combines morphological, physiologic and molecular phylogenetic analyses is used to characterize them. Numerous common pathogenic species, traditionally considered homogeneous, are indeed polyphyletic. Classifications of Candida albicans, Cryptococcus neoformans and Aspergillus fumigatus and relatives based on molecular phylogenetic analyses have been developed. The strains of C. albicans group are divided into 5 genotypes by PCR method. The strains of Cr. neoformans are divided into 3 varieties (var. grubii, var. neoformans, var. gattii) and into 5 serotypes. The strains of A.fumigatus and relative are divided into 5 clades based on DNA sequence analysis.
  • Takeshi Ouchi, Tomotaka Sato, Naho Yoshizawa, Makoto Sugiura, Keisuke Nagao, Takashi Yaguchi, Yasuki Hata
    Japanese Journal of Medical Mycology 49(2) 119-123 2008年  
    Pseudallescheria boydii is a ubiquitous filamentous fungus. We report a case of cutaneous P. boydii infection of the left knee in a 79-year-old Japanese man who was receiving oral predonisolone (25 mg/day) for radiation pneumonitis after radiation therapy on left breast cancer. He presented with a 2-week-history of a lesion on the left knee. A biopsy specimen from the skin lesion revealed granulomatous inflammation with hyphae. Culture of the pus from the skin specimen confirmed the diagnosis of cutaneous P. boydii infection. rDNA ITS sequence was analyzed to confirm the mycological diagnosis. The patient was treated orally with 200 mg/day of itraconazole. The lesion was gradually cured and left a hypertrophic scar. Cutaneous injury may be responsible for an incidence of localized infection. Such rare fungus infection in immunocompromised patients who have a persistent traumatic skin ulcer needs to be ruled out. An opportunistic infection in immunocompromised patients can be life-threatening and prompt treatment based on accurate diagnosis is important.
  • Nobuaki Kushida, Naoko Watanabe, Takako Okuda, Furnikazu Yokoyama, Yasuhiro Gyobu, Takashi Yaguchi
    JOURNAL OF ANTIBIOTICS 60(11) 667-673 2007年11月  
    Three novel histamine H3 receptor (H3R) ligands, PF1270A (1), PF1270B (2) and PF1270C (3) were isolated from the culture broth of the fungal strain PF1270. The strain was identified as Penicillium waksmanii on the basis of morphological characteristics. These compounds were obtained from the culture broth by solvent extraction and chromatographic purification. Their structures were established by spectroscopic methods and X-ray crystallographic analysis. They possess pentacyclic spiroindolinone skeletons. 1, 2 and 3 displayed high affinity for the rat H3R (Ki=0.058, 0.17 and 0.19 mu M, respectively) and human H3R (Ki=0.047, 0.12 and 0.22 mu M, respectively). Moreover, 1, 2 and 3 acted as potent agonists with the EC50 values of 0.12, 0.15 and 0.20 mu M, respectively.
  • Shou Nakadate, Koohei Nozawa, Hitoshi Horie, Yuichi Fujii, Masahiro Nagai, Tomoo Hosoe, Ken-Ichi Kawai, Takashi Yaguchi, Kazutaka Fukushima
    JOURNAL OF NATURAL PRODUCTS 70(9) 1510-1512 2007年9月  
    Three new decalin derivatives, eujavanicols A-C (1-3), were isolated from an extract of Eupenicillium javanicum IFM 54704. Their structures were determined by chemical and spectroscopic methods.
  • Kyoko Yarita, Ayako Sano, Yoshiteru Murata, Akiko Takayama, Yoko Takahashi, Hideo Takahashi, Takashi Yaguchi, Akira Ohori, Katsuhiko Kamei, Makoto Miyaji, Kazuko Nishimura
    MYCOPATHOLOGIA 164(3) 135-147 2007年9月  
    Four strains of Ochroconis gallopava from 3 out of 15 Japanese hot springs were isolated. Colonies of the hot spring isolates were uniformly floccose and dark olive green on the surface and dark brown on their reverse side on potato dextrose agar (PDA) plates, however, they became felty, flat, and brownish-black, and produced a reddish-brown pigment after several times of subculture at room temperature. Shapes and sizes of conidia of the four strains were individual, while the D1/D2 domain of the large subunit ribosomal RNA gene sequences showed 99.7% identity in the GenBank database. The DNA pattern of the hot spring isolates amplified by species specific loop mediated isothermal amplification method were as the same pattern as that of a clinical isolate. The minimum inhibitory concentrations of antifungal agents to O. gallopava isolated from the hot springs were ranged from 0.5 to 1 mu g/ml in amphotericin B, 1 to 16 mu g/ml in flucytosine, 0.125 to 0.25 mu g/ml in itraconazole, 1 to 4 mu g/ml in miconazole, 16 to 64 mu g/ml in flconazole and 0.03 to 0.5 mu g/ml in micafungin. The isolates had fatal outcome in experimentally infected mice intravenously with severe invasiveness to brains and kidneys. These findings suggested that O. gallopava habitats in hot springs could be one of sources for infection.
  • Kiyoshi Ishii, Masahito Hitosugi, Takashi Yaguchi, Shogo Tokudome
    Legal Medicine 9 287 2007年9月1日  
  • 矢口 貴志, 亀井 克彦, 三上 襄
    細胞工学 26(9) 1066-1069 2007年8月  
  • Takashi Yaguchi, Reiko Tanaka, Kazuko Nishimura, Shun-ichi Udagawa
    MYCOSES 50(4) 255-260 2007年7月  
    A phylogenetic study based on sequence analysis of the ribosomal internal transcribed spacer (ITS) 1, 5.8S and ITS2 region was performed in 33 strains morphologically identified as Fonsecaea pedrosoi from clinical specimens and living environments, preserved at the Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University. The strains of Fonsecaea were divided into two major clades: Group A, representing F. pedrosoi sensu de Hoog et al.; and Group B, representing F. monophora. We identified new lineages: Group B was further clustered into three subgroups, B-1, B-2 and B-3, which were phylogenetically distinct from each other. The subgroups B-1, B-2 and B-3 are thought to be mainly predominant in Africa and Central and South America, Japan and China, and Europe and North America, respectively. Thus, polymorphism in Group B is rather considered to be caused by its geographic parameter. We demonstrate for the first time that all clinical isolates from Japan are monophyletic, belonging to a new lineage of F. monophora.
  • S. A. Balajee, J. Houbraken, P. E. Verweij, S-B. Hong, T. Yaghuchi, J. Varga, R. A. Samson
    STUDIES IN MYCOLOGY 59(59) 39-46 2007年  
    Multiple recent studies have demonstrated the limited utility of morphological methods used singly for species identification of clinically relevant aspergilli. It is being increasingly recognised that comparative sequence based methods used in conjunction with traditional phenotype based methods can offer better resolution of species within this genus. Recognising the growing role of molecular methods in species recognition, the recently convened international working group meeting entitled "Aspergillus Systematics in the Genomic Era" has proposed several recommendations that will be useful in such endeavors. Specific recommendations of this working group include the use of the ITS regions for inter section level identification and the P-tubulin locus for identification of individual species within the various Aspergillus sections.
  • Takashi Yaguchi, Yoshikazu Horie, Reiko Tanaka, Tetsuhiro Matsuzawa, Junko Ito, Kazuko Nishimura
    Japanese Journal of Medical Mycology 48(1) 37-46 2007年  
    A phylogenetic study based on sequence analysis of the β-tubulin, hydrophobin and calmodulin genes was performed in 19 strains of Aspergillus fumigatus and related species isolated from clinical specimens in Japan. Correlations between detailed morphology and phylogeny were examined. Species in the section Fumigati were divided into five clades: clade I, typical strains of A. fumigatus clade II, species including A. lentulus and A. fumisynnematus clade III, species including A. fumigatiaffinis and A. novofumigatus, clade IV, atypical strains of A. fumigatus including A. viridinutans and clade V, species including A. brevipes, A. duricaulis and A. unilateralis. Most of the examined strains from clinical specimens in Japan clustered together in clade I and exhibit globose conidia with lobatereticulate ornamentation. Other strains from clinical specimens were divided into two clades (clades II and IV). The strains in clades II and the six strains in clade IV exhibit conidia with microtuberculate ornamentation, while A. viridinutans-complex in clades IV and the strains in clade V have conidia with lobate-reticulate ornamentation. The six strains are clearly distinguished from A. viridinutans-complex and are considered to be related to Neosartorya udagawae. The maximal growth temperatures of clades I, II, IV and V were above 50°C, 45°C, 42°C and 42°C, respectively. These data are useful for classification of species within the Aspergillus section Fumigati.
  • Takashi Yaguchi, Kyoko Takizawa, Hideaki Taguchi, Reiko Tanaka, Tadashi Kubota, Yoshiaki Kubota, Masaaki Kubota, Kazutaka Fukushima
    Japanese Journal of Medical Mycology 48(2) 97-100 2007年  
    Silver has long been known to have an antimicrobial activity against bacteria and other microorganisms, and has been used as eating utensils, as dental fillings and so on. We developed a novel adduct, GX-95, of silver with nanometer-scale particles to peptidic hydrolysates from collagen. Antifungal activity of the adduct against pathogenic yeasts and filamentous fungi was examined in terms of minimal inhibitory concentrations (MICs). GX-95 was found to possess strong and broad antifungal activities against all fungi examined in the following MICs: 0.25 to 0.31 μg/ml for Candida albicans including resistant strains to fluconazole, itraconazole and flucytosine, 0.05 to 0.2 μg/ml for Cryptococcus neoformans strains, 0.025 to 0.4 μg/ml for Aspergillus fumigatus strains, 0.4 μg/ml for Trichophyton rubrum, and 0.05 μg/ml for Cladophialophora carrionii.
  • Ahmed Hanafy, Junji Ito, Soji Iida, Yingqian Kang, Takahisa Kogure, Katsukiyo Yazawa, Takashi Yaguchi, Yuzuru Mikami
    MYCOPATHOLOGIA 162(4) 281-287 2006年10月  
    In Japan during 1996-2004, 21 actinomycete strains that have madurose as the diagnostic cell-wall sugar and show true branching in their substrate and aerial mycelia were isolated from sputa or bronchoalveolar lavage (BAL) fluid of patients with pulmonary infections or who were suspected of having related infections. Chemotaxonomic studies showed that all the isolates belong to the genus Actinomadura. Among them, six and seven strains were classified respectively into clusters of Actinomadura nitritigenes and Actinomadura cremea based on 16S rDNA analyses because their 16S rDNA similarities to those respective species were greater than 99.5%. To our knowledge, this is first report that strains of above two species were isolated from clinical specimens. Neither Actinomadura madurae nor Actinomadura pelletieri strain was isolated, and one new species, Actinomadura chibensis, was proposed; the remaining seven strains were not assigned into any known species, suggesting the presence of another new Actinomadura species.
  • Shou Nakadate, Koohei Nozawa, Hitoshi Horie, Yuichi Fujii, Masahiro Nagai, Shin-ichirou Komai, Tomoo Hosoe, Ken-ichi Kawai, Takashi Yaguchi, Kazutaka Fukushima
    HETEROCYCLES 68(9) 1969-1972 2006年9月  
    Two new dioxomorpholine derivatives, javanicunine A (1) and B (2) were isolated from the extract of Eupenicillium javanicum IFM 54704. These structures were determined by chemical and spectroscopic methods.
  • 高橋 英雄, 植田 啓一, 鎗田 響子, 村田 佳輝, Itano Eiko Nakagwa, 高山 明子, 猪股 智夫, 矢口 貴志, 佐野 文子, 西村 和子, 亀井 克彦
    日本医真菌学会雑誌 47(Suppl.1) 59-59 2006年8月  
  • HITOSUGI Masahito, ISHII Kiyoshi, YAGUCHI Takashi, CHIGUSA Yuichi, KUROSU Akira, KIDO Masahito, NAGAI Toshiaki, TOKUDOME Shogo
    Legal medicine 8(4) 240-242 2006年7月1日  
    We present a practical case in which the fungi have contributed to the estimation of postmortem interval of the cadaver. A 71-year-old man wearing a shirt and pants was found dead at the bottom of an open well about 6 m below the ground in his garden. Externally the face was dotted with colonies of white fungi, which was devoid of dipteran larvae and other insects. The fungi were identified as Penicillium sp. and Aspergillus terrous. These fungi, widely inhabit in the life circumstances, can generally colonize 3-7 days after attaching on the subjects. The appearance of the body surface, the state of decomposition of various organs, police information describing that the man had last been seen 12 days before discovery and the fungal evidence indicated that the man had been dead for about 10 days. We suggest that fungi can provide an useful means of estimating the minimum interval since death when forensic entomology is not applicable. Although, no data about growth rates of the fungi on human cadavers have been available, further researches should be needed to clarify the successive colonization of fungi on human cadavers. © 2006 Elsevier Ireland Ltd. All rights reserved.
  • MK Suh, JW Lim, YH Lee, GY Ha, H Kim, Kim, JR, T Yaguchi, K Nishimura
    BRITISH JOURNAL OF DERMATOLOGY 154(6) 1184-1189 2006年6月  
    We report the first case of subcutaneous hyalohyphomycosis caused by the genus Cephalotheca, which has not been reported to cause human infection. A 67-year-old immunocompetent farmer presented with a 10-year history of verrucous erythematous plaques on the right foot dorsum, great toe, heel and sole. Histopathology of the lesions revealed chronic granulomatous inflammation with numerous nonpigmented fungal spores in the dermis. Cultures of biopsy specimens on Sabouraud's dextrose agar for 2 weeks developed into yellowish brown, velvety colonies that subsequently turned black after 8 weeks because of the production of black cleistothecia indicating the teleomorph. On the basis of mycological features, scanning electron microscopic morphology and molecular analysis data, a new species of Cephalotheca was identified and designated Cephalotheca foveolata. Because there was no response to the antifungal agents administered, the patient was successfully treated by surgical excision with skin graft.
  • ISHII Kiyoshi, HITOSUGI Masahito, KIDO Masahito, YAGUCHI Takashi, NISHIMURA Kazuko, HOSOYA Tsuyoshi, TOKUDOME Shogo
    Legal medicine 8(3) 188-190 2006年5月1日  
    We examined and identified fungi flora found growing on two human cadavers. The main isolate colonized on the surfaces of the skin and bones was morphologically identified as Eurotium repens. The genus Eurotium usually inhabits soil and exhibits osmophilic properties. Because the cadavers contained abundant organic materials, Eurotium species formed ascomata and conidia. To our knowledge, this is the first study to describe in detail the species of fungus obtained from human cadavers. Because the detection of some species of fungus found in human cadavers may reveal the local habits of them, more forensic cases is needed to develop fungi into the forensic tool. © 2006 Elsevier Ireland Ltd. All rights reserved.
  • Takashi Yaguchi, Ayako Sano, Kyoko Yarita, Moo Kyu Suh, Kazuko Nishimura, Shun-ichi Udagawa
    MYCOTAXON 96 309-322 2006年4月  
    The causative agent isolated from a Korean patient with subcutaneous fungal infection is described as Cephalotheca foveolata, a new species of Cephalotheca, Cephalothecaceae, Ascomycota. It is characterized by globose cleistothecia, covered with yellow to brown hairs, cephalothecoid peridium, prototunicate asci, broadly reniform and foveolate ascospores, and a Phialemonium-like anamorph. Based on the analysis of sequences of the D1/D2 region of 28S rDNA, the fungus is phylogenetically a member of Cephalotheca. This is a new record of the genus from a patient with a human mycosis.
  • 田中 玲子, 伊藤 純子, 知花 博治, 三上 襄, 西村 和子, 矢口 貴志
    日本医真菌学会総会プログラム・抄録集 50 24-24 2006年  査読有り
    近年の分子生物学的解析の進歩は生物種の系統分類体系を大きく変貌させてきた.古典的な分類・同定は,形態学的あるいは生理学的な手法により行われてきたが,分子生物学的手法を用いることで DNA の塩基配列の相違などから,形態学的,生理学的にはほとんど同一にみえる生物種であってもさらにサブグループに分類することが可能となった.臨床の場面においては感染症の原因菌について,同定はもちろん感染経路などを探索する場合に,このサブグループの情報が非常に有効であることが知られている.本研究では,病原性酵母 Candida albicans について 2 種類の指標(25S genotype と ALTS)を用いて検討した.<BR>方法:千葉大学真菌医学研究センター保存の C. albicans 1110 株について McCullough 等1) の方法(25S genotype)による A,B,C のタイプ分けと Chibana 等2) の方法を一部改良した ALTS によるタイプ分けの後,分離源情報とを合わせ検討した.<BR>結果:分離源情報の内,分離国と分離源が明確な株についての比較により以下の点が明らかになった.日本,ブラジル,タイの 3 カ国の比較ではタイにおいて特徴的なタイプの分布割合を示した.分離源においては,AIDS 患者の血液等の内臓由来の菌株で genotype B が高くなっていることが判明した.<BR>1):McCullough MJ et al.: J Clin Microbiol 37: 417-421, 1999.<BR>2):Chibana H et al.: J Bacteriol 176: 3851-3858, 1994.
  • Wakamoto D, Hosoe T, Itabashi T, Nozawa K, Okada K, Takaki GC, Yaguchi T, Takizawa K, Fukushima K, Kawai K
    Mycotaxion 56(1) 3-6 2006年  
  • Hosoe T, Wakamoto D, Itabashi T, Nozawa, Okada K, Takaki GC, Takizawa K, Yaguchi T, Takizawa K, Fukushima K, Kawai K
    Mycotaxion 56(1) 7-10 2006年  
  • Shin-ichirou Komai, Tomoo Hosoe, Takeshi Itabashi, Koohei Nozawa, Takashi Yaguchi, Kazutaka Fukushima, Ken-ichi Kawai
    JOURNAL OF NATURAL MEDICINES 60(3) 185-190 2006年  
    Two new penicillide derivatives, secopenicilides A (3) and B (4), were isolated along with penicillide (1) and purpactin A (2), and altenusin (5) and dehydroaltenusin (6), the antifungal substances of this fungus, from the extract of Penicillium simplicissimum IFM 53375. The absolute structures of 3 and 4 were determined by spectroscopic investigation and chemical correlation to penicillide (1). The absolute configuration of purpactin A (2) was determined by the chemical method.
  • Daigo Wakana, Tomoo Hosoe, Takeshi Itabashi, Kaoru Okada, Galba Maria de Campos Takaki, Takashi Yaguchi, Kazutaka Fukushima, Ken-ichi Kawai
    JOURNAL OF NATURAL MEDICINES 60(4) 279-284 2006年  
    Three new coupling compounds derived from citrinin (9) and 2,3,4-trimethyl-5,7-dihydroxy2,3-dihydrobenzofuran (8) - penicitrinone A (1), penicitrinol A (2) and penicitrinone B (3) - and a new citrinin derivative - decarboxydihydrocitrinin (4) - were isolated along with 3-methoxy-1-methyl-4(1H)-quinolone (5), quinolactacin A2 (6), dihydrocitrinone (7), 2,3,4-trimethyl-5,7-dihydroxy-2,3-dihydrobenzofuran (8), citrinin (9), quinocitrinin A or B (10), and decarboxydihydrocitrinone (11) from the extract of Penicillium citrinum IFM 53298. The relative structures of compounds 1-4 were confirmed on the basis of spectroscopic investigations and chemical correlations. Citrinin (9) and quinolactacin A2 (6) both showed antifungal activity.
  • S Komai, T Hosoe, T Itabashi, K Nozawa, T Yaguchi, K Fukushima, K Kawai
    HETEROCYCLES 65(11) 2771-2776 2005年11月  
    Four new vermistatin derivatives, dihydrovermistatin (1), acetoxy-dihydrovermistatin (2), hydroxydihydrovermistatin (3), and penisimplicissin (4) were isolated along with vermistatin (5), penicillide, and funicone from the extract of Penicillium simplicissimum IFM 53375. The structures of 1 - 4 were determined by spectroscopic and chemical methods.
  • S Komai, T Hosoe, T Itabashi, K Nozawa, K Okada, GMD Takaki, T Yaguchi, K Takizawa, K Fukushima, K Kawai
    CHEMICAL & PHARMACEUTICAL BULLETIN 53(9) 1114-1117 2005年9月  
    In the course of our rsearch for new antifungal agents, two new meroterpenoids, penisimplicin A (1) and B (2), were isolated from Penicillium simplicissimum. The absolute structures of 1 and 2 were established by spectroscopic and chemical investigation. Penisimplicin A (1) and B (2) are rare examples of D-ring seco-meroterpenoids. Since compounds 1 and 2 showed no antifungal activities, the isolation of the compounds with antifungal activity will be attempted.
  • 西村 和子, 鎗田 響子, 大堀 陽, 高橋 容子, 矢口 貴志, 村田 佳輝, 佐野 文子, 亀井 克彦
    日本医真菌学会雑誌 46(Suppl.1) 75-75 2005年8月  
  • T Yaguchi, S Udagawa, K Nishimura
    CRYPTOGAMIE MYCOLOGIE 26(2) 133-141 2005年6月  
    Talaromyces eburneus, previously unregarded as a heat resistant fungus, is redescribed on new isolate from a spoilage outbreak that involved a pasteurized pineapple juice in Japan. Based on examination of the new isolate, type studies, and the D1/D2 region of 28S rDNA sequence analysis, we conclude that Geosmithia argillacea is assigned to the teleomorphic species T eburneus as an anamorph. Heat resistant fungus identification such as this finding is important in the study of food spoilage.
  • S Okamoto, T Hosoe, T Itabashi, K Nozawa, K Okada, GMD Takaki, M Chikamori, T Yaguchi, K Fukushima, M Miyaji, K Kawai
    JOURNAL OF NATURAL PRODUCTS 67(9) 1580-1583 2004年9月  
    Two new decalin derivatives, eujavanoic acids A (1) and B (2), were isolated from Eupenicillium javanicum, along with several compactin derivatives. The structures of 1 and 2 were determined by spectroscopic methods and modified Mosher's method. The side chain (2-methylbutanoyloxy) and acid functionalities of compactin derivatives were necessary to show the antifungal activity.
  • T Kuriyama, E Kakemoto, N Takahashi, KI Imamura, K Oyama, E Suzuki, K Harimaya, T Yaguchi, Y Ozoe
    JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 52(12) 3884-3887 2004年6月  
    The gamma-aminobutyric acid (GABA) receptor bears sites of action for insecticides. To discover GABA receptor-directed insecticides in natural products, fungal culture extracts were screened for their ability to inhibit specific binding of the radiolabeled noncompetitive antagonist [(3)H]1 -(4-ethynylphenyl)-4-n-propyl-2,6,7-trioxabicyclo[2.2.2]octane to housefly head membranes. The screening efforts led to the isolation of two alkaloids from Aspergillus terreus: PF1198A(alantrypinone) and PR1198B (seran-trypinone), which had IC(50) values of 0.34 and 2.1 muM, respectively, in this assay. These compounds were ca. 47-61-fold selective for housefly vs rat GABA receptors. Both compounds showed insecticidal activity against Myzus persicae in the range of 100-500 ppm. Binding assay-guided screening should provide significant opportunities for the identification of novel and selective insecticides.
  • 駒井 信一郎, 細江 智夫, 板橋 武史, 野沢 幸平, オカダ カオル, デ・カンポス・タカキ ガルバ, 近森 穣, 矢口 貴志, 福島 和貴, 宮治 誠, 河合 賢一
    Mycotoxins 54: 15-19(1) 15-19 2004年  
    深在性真菌症に対する新規抗真菌剤の開発の一環として,土壌由来の分離菌102 株を,病原性糸状菌2 種および病原性酵母2 種に対する抗真菌試験を指標に探索を行い,37 菌株のエキスに何らかの抗真菌活性が認められた.そこで,日和見感染症の一つであるアスペルギルス症などの原因菌であるアスペルギルス・フミガタスに対して特異的に抗真菌活性を示したタラロマイセス・フラブス IFM52668 株に着目し,その成分検索を行った.その結果,フニコン(1),ベルミスタチン(3)およびエメリン(4)とともに新規化合物9,14- エポキシ-11-デオキシフニコン(2) を単離することができた.化合物2 は各種スペクトル,特にNMR スペクトルの解析から,その平面構造を決定した.また化合物1,3 および4 についても各種スペクトルの解析から,その構造を決定した.これらの化合物の抗真菌テストの結果,主成分であるフニコン(1)が弱いながら抗アスペルギルス・フミガタス活性を示したことより,タラロマイセス・フラブス IFM52668 株の抗真菌活性本体はフニコン(1)であることが明らかとなった.
  • Y Ozoe, T Kuriyama, Y Tachibana, K Harimaya, N Takahashi, T Yaguchi, E Suzuki, KI Imamura, K Oyama
    JOURNAL OF PESTICIDE SCIENCE 29(4) 328-331 2004年  
    For the purpose of discovering GABA receptor-directed insecticides in natural products, fungal culture extracts were screened for their ability to inhibit the specific binding of the noncompetitive antagonist [H-3]EBOB to housefly head membranes. The screening efforts led to the isolation of a derivative of dihydroisocoumarin (PF1223) from the culture of Neosartorya quadricincta. This compound at 2.2 muM inhibited [H-3]EBOB binding by 65%. This ligand might prove to be a lead compound for the identification of novel insecticides acting at the insect GABA receptor. (C) Pesticide Science Society of Japan.
  • Koichiro Murashima, Tomoko Nishimura, Yuko Nakamura, Jinichiro Koga, Tastuki Moriya, Naomi Sumida, Takashi Yaguchi, Toshiaki Kono
    Enzyme and Microbial Technology 30(3) 319-326 2002年3月  
    New extracellular endoglucanases, designated RCE1 and RCE2, produced by Rhizopus oryzae isolated from the soil, were purified to apparent homogeneity from the culture supernatant. The molecular mass of RCE1 and that of RCE2 were found to be 41 kDa and 61 kDa, respectively. The N-terminal amino acid sequences of RCE1 and RCE2 showed high homology with those of the family I cellulose-binding domains. Internal amino acid sequences of RCE1 and RCE2 showed homology with that of the catalytic domain of EGV from Humicola insolens belonging to family 45 endoglucanase. The cellooligosaccharide hydrolysis patterns of RCE1 and RCE2 were similar to that of EGV from H. insolens. These results indicate that RCE1 and RCE2 are family 45 endoglucanases having a cellulose binding domain at their N-terminus. RCE1 and RCE2 hydrolyzed carboxymethylcellulose (CMC), insoluble cellooligosaccharide (G33), cellohexaose, and cellopenpaose, but not Avicel, xylan, galactan, arabinan, mannan, or laminarin. The CMCase activity of both enzymes was inhibited by Cu2+, Zn2+, Co2+, and Pb2+. The optimum pH for the CMCase activity of both enzymes was found to be between pH value 5.0 and 6.0, and the optimum temperature was 55°C, the lowest among the family 45 endoglucanases. These results indicate that RCE1 and RCE2 represent a new type of endoglucanases having the lowest optimum temperature among the family 45 endoglucanases. © 2002 Elsevier Science Inc. All rights reserved.
  • S Suzuki, T Hosoe, K Nozawa, K Kawai, T Yaguchi, S Udagawa
    JOURNAL OF NATURAL PRODUCTS 63(6) 768-772 2000年6月  
    The dichloromethane extract of Talaromyces convolutus cultivated on barley exhibited antifungal activity against Candida albicans. In the course of a search for the active compounds, four new tetramic acid derivatives, talaroconvolutins A (1), B (2), C (3), and D (4), were isolated along with ZG-1494 alpha (5), and mitorubrin derivatives. The structures of talaroconvolutins A-D (1-4) were established on the basis of spectroscopic and chemical investigations and chemical correlations. The antifungal activity of the talaroconvolutins against the pathogenic fungi Aspergillus fumigates, Aspergillus niger, C. albicans, and Cryptococcus neoformans was determined.
  • H Nose, A Seki, T Yaguchi, A Hosoya, T Sasaki, S Hoshiko, T Shomura
    JOURNAL OF ANTIBIOTICS 53(1) 33-37 2000年1月  
    Two novel antifungal antibiotics, PF1163A and B, were isolated from the fermentation broth of Penicillium sp. They were purified from the solid cultures of rice media using ethyl acetate extraction, silica gel and Sephadex LH-20 column chromatographies. PF1163A and B showed potent growth inhibitory activity against pathogenic fungal strain Candida albicans but did not show cytotoxic activity against mammalian cells. These compounds inhibited the er,ergosterol biosynthesis in Candida albicans.
  • Ayako Someya, Takashi Yaguchi, Shun-Ichi Udagawa
    Mycoscience 40(5) 405-409 1999年10月15日  
    A new species of Neosartorya, N. sublevispora (anam. Aspergillus sublevisporus), is described and photographed. The species is characterized by non-ostiolate ascomata covered loosely with a pale yellowish hyphal envelope, lenticular ascospores with two low equatorial crests, and subglobose to ellipsoidal, microtuberculate conidia. The ornamentation of ascospores, which is composed of two closely appressed crests and small even-sized echines on their convex surfaces, particularly serves to distinguish this species from other recognized species. A revised key to all accepted species of the genus is provided.
  • S Suzuki, T Hosoe, K Nozawa, T Yaguchi, S Udagawa, K Kawai
    JOURNAL OF NATURAL PRODUCTS 62(9) 1328-1329 1999年9月  
    Two groups, producers of (+)- and (-)-mitorubrin derivatives, coexist in the series Lutei of the genus Talaromyces. The optical rotations of mitorubrins From T. emodensis, T. hachijoensis, and T. wortmannii var, sublevisporus, which produced mitorubrinol acetate (5), were all positive, whereas those from T. austrocalifornicus and T. convolutus, which produced mitorubrinal (3) and mitorubrinic acid (4), were all negative.
  • Takashi Yaguchi, Ayako Someya, Shun Ichi Udagawa
    Mycoscience 40(2) 133-136 1999年4月15日  
    A new species of Talaromyces, characterized by development of unusual deep green ascomata on common media, is described and given the name Talaromyces euchlorocarpius. This species, isolated from soil, also produces ellipsoidal, spinose ascospores, typically biverticillate penicilli, large ellipsoidal, smooth-walled conidia, and is assigned to the series Lutei of the section Talaromyces.
  • Yuji Tabata, Shuji Ikegami, Takashi Yaguchi, Toru Sasaki, Shigeru Hoshiko, Sadatoshi Sakuma, Kazuo Shin-Ya, Haruo Seto
    Journal of Antibiotics 52(4) 412-414 1999年  
  • Takashi Yaguchi, Ayako Someya, Shun-Ichi Udagawa
    Mycoscience 38(1) 83-85 1997年4月15日  
    Roumegueriella rufula (Ascomycota Hypocreales) isolated from a soil sample collected in Taiwan is described as a new record from East Asia. Some observations are recorded, particularly on cultural characteristics of a Gliocladium anamorph of this fungus, which has scarcely been reported since Bainier described both ascosporic and conidial forms as species of Gliocladium.
  • Yuji Tabata, Naoko Miike, Masahiro Hatsu, Yasushi Kurata, Takashi Yaguchi, Ayako Someya, Shinji Miyadoh, Shigeru Hoshiko, Takashi Tsuruoka, Shoji Omoto
    Journal of Antibiotics 50(4) 304-308 1997年  
    Three new nonsteroidal progesterone receptor ligands, PF1092A, B and C, have been isolated from Penicillium oblatum. They were purified from the solid cultures of rice media using ethyl acetate extraction, silica gel and Sephadex LH-20 column chromatographies, and crystallization. All three ligands competitively inhibited [3H]-progesterone binding to porcine uteri cytosol preparations with IC50 of 3.0 x 10 nM (PF1092A), 2.2 x 102 nM (PF1092B) and 2.2 x 103 nM (PF1092C).

MISC

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  • 馬目照久, 中村文香, 原康雅, 原康雅, 高屋明子, 高屋明子, 高屋明子, 伴さやか, 高橋弘喜, 高橋弘喜, 矢口貴志, 石橋正己, 石橋正己
    日本薬学会年会要旨集(Web) 143rd 2023年  
  • 三宅真未, 原康雅, 原康雅, 渡邊圭一郎, 高屋明子, 高屋明子, 高屋明子, 矢口貴志, 石橋正己, 石橋正己
    日本薬学会年会要旨集(Web) 143rd 2023年  
  • 原康雅, 渡邊圭一郎, 高屋明子, 高屋明子, 馬目照久, 矢口貴志, 石橋正己
    日本生薬学会年会講演要旨集 68th 2022年  
  • 井上 幸次, 宮崎 大, 馬場 高志, 石倉 涼子, 松浦 一貴, 長谷川 晶子, 諸星 計, 小松 恵子, 小松 直樹, 唐下 千寿, 寺坂 祐樹, 富長 岳史, 三原 幸子, 宮野 佐智子, 佐々木 慎一, 川本 由紀美, 春木 智子, 井上 美智子, 大谷 史江, 稲田 耕大, 魚谷 竜, 清水 大輔, 三宅 瞳, 江原 二三枝, 郭 權慧, 矢倉 慶子, 清水 由美子, 大橋 裕一, 下村 嘉一, 臼井 正彦, 姚 玉峰, 外園 千恵, 井上 智之, 鈴木 崇, 加藤 四郎, 上田 重晴, 前田 一洋, 佐々木 裕美, 坂本 雅子, 山田 雅夫, 林 皓三郎, 塩田 洋, 綾塚 祐二, 佐々木 香る, 浅利 誠志, 砂田 淳子, 木村 圭吾, 福田 昌彦, 江口 洋, 秦野 寛, 細貝 真弓, 山上 聡, 海老原 伸行, 鈴木 弘, 星 最智, 横倉 俊二, 望月 清文, 槇村 浩一, 矢口 貴志, 錫谷 達夫, 小杉 伊三夫, 門田 遊, 子島 良平, 八木 彰子, 古川 敏仁
    日本眼科学会雑誌 124(3) 155-184 2020年3月  
    眼感染症と他の眼疾患の大きな違いは,他の眼疾患にはホストと環境の二つが関連しているが,眼感染症はホストと微生物と環境の三者が絡み合って疾患を形成してくることにある.それだけにより複雑であり,それを読み解き,解決を図っていくためには,さまざまな手法を駆使する必要がある.本論文では,我々が基礎から臨床までさまざまな方法で眼感染症に取り組んできたこと,取り組んでいることを紹介する.1.角膜ヘルペス Herpes simplex virus(HSV)による角膜炎はウイルスの増殖とウイルスに対する免疫反応との微妙なバランスで成り立っており,いまだ根本的な解決はなされておらず,臨床的な重要性は高い.そしてそれらの解決に向けて基礎研究の積み重ねが重要で,そこには最新の研究手法が応用できる.in vitroでは,角膜上皮細胞・角膜内皮細胞にHSVを感染させてmRNAをみるマイクロアレイで網羅的に発現遺伝子を解析し,その結果,重要性が判明した遺伝子の中で,interferon regulatory factor 7(IRF7)やindoleamine 2,3-dioxygenase 1(IDO1)のHSVに対する免疫反応への関与を解析してきた.in vivoではヘルペス制御に対するワクチンの試みやケモカイン受容体と角膜実質炎の関係,新しい抗ヘルペス薬アメナメビルの効果の可能性をマウスのHSV角膜炎モデルで検討してきた.臨床への研究手法の応用としてreal-time polymerase chain reaction(PCR)による診断を行ってきた.2.サイトメガロウイルス角膜内皮炎 2006年の小泉らの報告以来多くの研究・報告がなされているが,その病態はいまだ不明である.我々も臨床例における前房内のサイトメガロウイルス(CMV)-DNA量が種々の臨床的パラメーターと相関していること,CMVが角膜内皮細胞や線維柱帯細胞に実際に感染し,サイトカイン産生を含め,さまざまな反応を惹き起こしてくること,CMV感染角膜内皮細胞によるCD8陽性細胞傷害性T細胞誘導がCMV角膜内皮炎患者では低下していることなどを見出してきた.3.真菌性角膜炎多施設スタディ 日本眼感染症学会においてプロスペクティブな多施設観察研究として,起因真菌とその薬剤感受性・臨床所見や予後に関与する因子を検討した.多種多様な真菌が原因となっていることが,遺伝子解析で明らかとなり,in vitroの感受性検査では,ピマリシンとアゾール系の組み合わせで拮抗を示す株もあることが判明した.4.角膜感染症への人工知能応用の試み 現在多くの眼疾患への人工知能(AI)の応用が試みられており,今後日本眼科学会主導でAI研究が行われていくが,我々もImageNetデータベースの一般画像を用いて事前学習させた畳み込みニューラルネットワークというアルゴリズムに感染性角膜炎の細隙灯顕微鏡写真を学習させ,比較的良好な診断確度を得つつある.また,それと並行して細隙灯顕微鏡写真以外のデータ(培養・real-time PCR)を用いた診断モデルの構築もランダムフォレストなど別のアルゴリズムを用いて行っている.将来的にはこれを統合させることでAIによる診断が可能になると思われる.5.術後眼内炎予防 白内障術後眼内炎の予防についてはさまざまな試みが行われている.日本眼感染症学会主導の多施設スタディにおいて手術3日前からの抗菌点眼薬使用によって結膜嚢常在菌を減らすことができることを証明したが,その後ヨード製剤の術中使用や抗菌薬の前房内投与が行われるようになってきたこと,耐性菌対策が重要となってきたことなどから,術前抗菌点眼薬使用を見直す必要が出てきていると思われる.我々は術中ヨード製剤の使用によって,結膜嚢常在菌が減ること,また,術中ヨード製剤の使用が術前抗菌点眼薬使用に劣らない減菌効果を示すことをプロスペクティブスタディで証明した.これまで,眼感染症に関するさまざまな問題に対して研究が行われ,我々もそれに継続的に参画してきたが,今後も眼感染症は眼科臨床において重要な問題を提起し続けると思われ,最新の医学を貪欲に取り入れつつ,基礎から臨床にわたる幅広いアプローチでこの古くて新しい脅威に立ち向かっていく必要がある.(著者抄録)
  • 北原 博一, 稲本 伸子, 本田 治樹, 矢口 貴志, 佐藤 友隆
    臨床皮膚科 73(6) 435-439 2019年5月  
    <文献概要>77歳,男性.眉毛部の紅斑を主訴に受診.ステロイド外用にて治療開始したが,紅斑が増悪した.眉毛のKOH直接鏡検より菌糸を認め,顔面白癬と診断した.妻にMicrosporum canisによる顔面白癬で,テルビナフィン内服による加療歴があり,また敷地内に野良猫が複数おり容易に接触できる環境であったためM. canisによる顔面白癬を疑った.一方で患者の足趾爪甲に白濁病変があり,鏡検にて菌糸を認め爪白癬と診断した.顔面白癬の原因菌を同定するため,眉毛および爪から培養したところ,同様の集落を形成し,菌学的検査にて両者ともTrichophyton rubrumと確定した.顔面白癬の感染経路として,動物や柔道・レスリング選手同士などでの感染が報告される一方で,爪白癬などによる自家播種も考慮すべきであり,感染源特定に培養同定検査が有用であった1例を報告する.

共同研究・競争的資金等の研究課題

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