研究者業績
基本情報
研究キーワード
2研究分野
1受賞
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2018年6月
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2016年9月
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2009年10月
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1996年
論文
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Mycoscience 37(2) 157-162 1996年7月15日Two striking fungi, isolated from soils from the island of Hachijo-jima in Japan, are described and illustrated. One of these represents e new species of Talaromyces (Ascomycetes), for which the name Talaromyces hachijoensis is proposed. The new species is distinctive among all other recognized species in its restricted growth on common media, yellow ascomata characterized by asci developing in chains, striate ascospores, and the absence of an anamorph. The other is Heterocephalum taiense, a rare hyphomycete which has only previously been recorded from Ivory Coast soil and probably from Panama soil.
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Mycoscience 37(3) 255-259 1996年Lasiobolidium gracile isolated from a soil sample collected from California, USA is described as a new species. This species is characterized by yellowish brown to reddish brown, nonostiolate ascomata with numerous, long straight appendages and a translucent peridium, subglobose to broadly clavate asci, hyaline to pale yellowish brown, ellipsoidal ascospores. It differs from the other species of Lasiobolidium in the straight and narrow appendages and the large ascospores. A key is presented separating the seven known species.
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Journal of Antibiotics 49 1996年1月1日
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Mycoscience 37(1) 55-60 1996年Ubiquinone profiles of 25 strains of Talaromyces were determined using reversed-phase high performance liquid chromatography. Together with data of earlier authors, examination of the most recently described species allowed us to solve some taxonomic problems in the intrageneric classification. Ubiquinone Q-10(H2) was found in the majority of Talaromyces species with a Penicillium anamorph, which are mostly placed in series Flavi and Lutei of section Talaromyces. Flavi and Lutei are closely related and homogeneous taxa based on their morphological characters used in the section. However, exceptional profiles were consistently shown by T. trachyspermus and the allied taxa of series Trachyspermi of the section Talaromyces, in which a mixture of Ubiquinone Q-10(H2) and Ubiquinone Q-10(H4) was found. Thus the accommodation of T. trachyspermus and the allied taxa in the section Talaromyces was not supported by their Ubiquinone systems. Trachyspermi is distinguished from other taxa of Talaromyces by its production of white ascomata and rapid growth at 37°C. A new section, Trachyspermus, is proposed herein. Section Emersonii showed heterogeneity the Ubiquinone profiles (Q-10(H2) or Q-10 and Q-10(H2)) in Talaromyces with a Geosmithia anamorph were relatively homogeneous, whereas those of another group with a Paecilomyces anamorph were considerably variable. Only Talaromyces with a Geosmithia anamorph can be placed in the section Emersonii.
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Mycoscience 36(2) 151-154 1995年A new variety of Thermoascus, Thermoascus crustaceus var. verrucosus, is described and illustrated. It is characterized by producing prominently verrucose ascospores. The other characters are almost identical with those of the type variety. Leucothecium coprophilum, a rare ascomycete that has only previously been reported from Spain, is recorded from soils in Taiwan and Korea. © 1995, Mycological Society of Japan. All rights reserved.
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Mycoscience 36(4) 421-424 1995年Aspergillus taichungensis isolated from a soil sample collected in Taiwan is described as a new species. The new species is characterized by its restricted growth on Czapek's and malt extract agars and its white to light yellow colonies, radiate conidial heads, smooth and often diminutive conidiophores, hemispherical to elongate vesicles with biseriate aspergilla (conidiogenous cells), globose, micro-verrucose conidia and dark brown sclerotia. The species somewhat resembles A. versicolor, A. terreus and A. flavipes, but differs in cultural and morphological details, and is considered to represent an interface species in the subgenus Nidulantes. © 1995, Mycological Society of Japan. All rights reserved.
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Mycoscience 35(3) 249-255 1994年Two new species of Talaromyces, isolated from soils in Taiwan and Japan, are described and illustrated. Talaromyces eburneus, associated with a Geosmithia anamorph, is characterized by off-white colony on oatmeal agar, small pale yellow ascomata, and subglobose to ovoid ascospores with a smooth wall. Talaromyces muroii is characterized by restricted growth on Czapek agar, luteous ascomata, which are initiated by paired gametangia like those seen in members of the series Flavi, ellipsoidal and nearly smooth ascospores, and the absence of an anamorph. © 1994, Mycological Society of Japan. All rights reserved.
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Mycoscience 35(1) 63-68 1994年A new variety of Talaromyces, T. wortmanniivar, sublevisporus, is described and illustrated. It is characterized by producing nearly smooth-walled ascospores. The other characters are almost identical with those of the type variety. Based on observations of several strains including the authentic ones, T. assiutensis is considered a finely rough-spored variety of T. trachyspermus. © 1994, Mycological Society of Japan. All rights reserved.
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Mycoscience 35(3) 219-222 1994年Erythrogymnotheca, a new genus of the Eurotiales, is proposed. The genus, based on the single species E. paucispora, is characterized by non-ostiolate ascomata with a telaperidium composed of an envelope of yellowish or reddish, branched, thin-walled hyphae, 1-2(-4)-spored asci, one-celled, subglobose to broadly ellipsoidal, thick-walled ascospores with spines, and the absence of an anamorph. The ascomatal initials of E. paucispora are long c|avate to vermiform, and reminiscent of those of Talaromyces flavus. © 1994, Mycological Society of Japan. All rights reserved.
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The Journal of Antibiotics 47(5) 571-580 1994年A new insecticidal compound PF1018 was isolated from the culture broth of Humicola sp. It exhibited insecticidal activity against a wide range of critical pest species. The structure of PF1018 was determined to be (7aS)-2-((2E)-l-hydroxy-3-((1S, 3aR, 4R, 5R, 7aR)-3a, 4, 5, 7a-tetrahydro-1, 3, 5, 7-tetramethyl-5, 1-((3S)-(Z)-2, 3-dimethylpropeno)-1H-inden-4-yl)-2-propenylidene)pyrrolizidine 1, 3-dione, by NMR spectral analyses coupled with X-ray crystallographic analysis and chemical degradation study. © 1994, JAPAN ANTIBIOTICS RESEARCH ASSOCIATION. All rights reserved.
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Mycoscience 35(2) 175-178 1994年Two interesting species of cleistothecial Ascomycetes with Aspergillus anamorphs are described: Fenneliia flavipes and Neosartorya stramenia, Both species were isolated from soil smaples collected in 1992 as a new record to Japan. © 1994, Mycological Society of Japan. All rights reserved.
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Mycoscience 35(4) 309-313 1994年A new species of Neosartorya, N. multiplicata (anam. Aspergillus multiplicatus), isolated from soil collected at Houli, Taichung, in Taiwan, is described and illustrated. The species is characterized by its restricted growth on Czapek agar, white ascomata, nearly globose ascospores with ribbed surface ornamentation of several linear ridges, and a limited development of conidia on common media. A key to all accepted species of the genus is provided. © 1994, Mycological Society of Japan. All rights reserved.
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JOURNAL OF ANTIBIOTICS 45(5) 692-697 1992年5月 査読有りThe novel anthelmintic cyclodepsipeptide PF1022A was isolated from cultured mycelia of Mycelia Sterilia PF1022 (FERM BP-2671). It showed strong anthelmintic activities against Ascaridia galli in chickens. The structure of PF1022A was determined to be cyclo(D-lactyl-L-N-methylleucyl-D-3-phenyllactyl-L-N-methylleucyl-D-lactyl-L-N-methylleucyl-D-3-phenyllactyl-L-N-methylleucyl) by spectroscopic analyses and chemical studies.
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JOURNAL OF FERMENTATION TECHNOLOGY 64(6) 473-479 1986年Intraspecific protoplast fusion was carried out between different citric acid-producing strains of Aspergillus niger. Protoplasts prepared from the mycelia of auxotrophic mutants were treated in 30% (w/v) polyethylene glycol solution containing 0.01 M CaCl2 and 0.5 M KCl, and fusion frequencies were 2.0-6.3%. Fusants were heterokaryons and in some cases formed sectors of the prototroph. Heterodiploids were induced from a heterokaryon by d-camphor treatment. Citric acid productivity of one heterodiploid was intermediate between those of parent strains in both shaking and solid cultures. © 1986.
MISC
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日本眼科学会雑誌 124(3) 155-184 2020年3月眼感染症と他の眼疾患の大きな違いは,他の眼疾患にはホストと環境の二つが関連しているが,眼感染症はホストと微生物と環境の三者が絡み合って疾患を形成してくることにある.それだけにより複雑であり,それを読み解き,解決を図っていくためには,さまざまな手法を駆使する必要がある.本論文では,我々が基礎から臨床までさまざまな方法で眼感染症に取り組んできたこと,取り組んでいることを紹介する.1.角膜ヘルペス Herpes simplex virus(HSV)による角膜炎はウイルスの増殖とウイルスに対する免疫反応との微妙なバランスで成り立っており,いまだ根本的な解決はなされておらず,臨床的な重要性は高い.そしてそれらの解決に向けて基礎研究の積み重ねが重要で,そこには最新の研究手法が応用できる.in vitroでは,角膜上皮細胞・角膜内皮細胞にHSVを感染させてmRNAをみるマイクロアレイで網羅的に発現遺伝子を解析し,その結果,重要性が判明した遺伝子の中で,interferon regulatory factor 7(IRF7)やindoleamine 2,3-dioxygenase 1(IDO1)のHSVに対する免疫反応への関与を解析してきた.in vivoではヘルペス制御に対するワクチンの試みやケモカイン受容体と角膜実質炎の関係,新しい抗ヘルペス薬アメナメビルの効果の可能性をマウスのHSV角膜炎モデルで検討してきた.臨床への研究手法の応用としてreal-time polymerase chain reaction(PCR)による診断を行ってきた.2.サイトメガロウイルス角膜内皮炎 2006年の小泉らの報告以来多くの研究・報告がなされているが,その病態はいまだ不明である.我々も臨床例における前房内のサイトメガロウイルス(CMV)-DNA量が種々の臨床的パラメーターと相関していること,CMVが角膜内皮細胞や線維柱帯細胞に実際に感染し,サイトカイン産生を含め,さまざまな反応を惹き起こしてくること,CMV感染角膜内皮細胞によるCD8陽性細胞傷害性T細胞誘導がCMV角膜内皮炎患者では低下していることなどを見出してきた.3.真菌性角膜炎多施設スタディ 日本眼感染症学会においてプロスペクティブな多施設観察研究として,起因真菌とその薬剤感受性・臨床所見や予後に関与する因子を検討した.多種多様な真菌が原因となっていることが,遺伝子解析で明らかとなり,in vitroの感受性検査では,ピマリシンとアゾール系の組み合わせで拮抗を示す株もあることが判明した.4.角膜感染症への人工知能応用の試み 現在多くの眼疾患への人工知能(AI)の応用が試みられており,今後日本眼科学会主導でAI研究が行われていくが,我々もImageNetデータベースの一般画像を用いて事前学習させた畳み込みニューラルネットワークというアルゴリズムに感染性角膜炎の細隙灯顕微鏡写真を学習させ,比較的良好な診断確度を得つつある.また,それと並行して細隙灯顕微鏡写真以外のデータ(培養・real-time PCR)を用いた診断モデルの構築もランダムフォレストなど別のアルゴリズムを用いて行っている.将来的にはこれを統合させることでAIによる診断が可能になると思われる.5.術後眼内炎予防 白内障術後眼内炎の予防についてはさまざまな試みが行われている.日本眼感染症学会主導の多施設スタディにおいて手術3日前からの抗菌点眼薬使用によって結膜嚢常在菌を減らすことができることを証明したが,その後ヨード製剤の術中使用や抗菌薬の前房内投与が行われるようになってきたこと,耐性菌対策が重要となってきたことなどから,術前抗菌点眼薬使用を見直す必要が出てきていると思われる.我々は術中ヨード製剤の使用によって,結膜嚢常在菌が減ること,また,術中ヨード製剤の使用が術前抗菌点眼薬使用に劣らない減菌効果を示すことをプロスペクティブスタディで証明した.これまで,眼感染症に関するさまざまな問題に対して研究が行われ,我々もそれに継続的に参画してきたが,今後も眼感染症は眼科臨床において重要な問題を提起し続けると思われ,最新の医学を貪欲に取り入れつつ,基礎から臨床にわたる幅広いアプローチでこの古くて新しい脅威に立ち向かっていく必要がある.(著者抄録)
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臨床皮膚科 73(6) 435-439 2019年5月＜文献概要＞77歳,男性.眉毛部の紅斑を主訴に受診.ステロイド外用にて治療開始したが,紅斑が増悪した.眉毛のKOH直接鏡検より菌糸を認め,顔面白癬と診断した.妻にMicrosporum canisによる顔面白癬で,テルビナフィン内服による加療歴があり,また敷地内に野良猫が複数おり容易に接触できる環境であったためM. canisによる顔面白癬を疑った.一方で患者の足趾爪甲に白濁病変があり,鏡検にて菌糸を認め爪白癬と診断した.顔面白癬の原因菌を同定するため,眉毛および爪から培養したところ,同様の集落を形成し,菌学的検査にて両者ともTrichophyton rubrumと確定した.顔面白癬の感染経路として,動物や柔道・レスリング選手同士などでの感染が報告される一方で,爪白癬などによる自家播種も考慮すべきであり,感染源特定に培養同定検査が有用であった1例を報告する.
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